Increased interest in using zebrafish as a model organism has led to a resurgence of fin regeneration studies. This has allowed for the identification of a large number of gene families, including signaling molecules and transcription factors, which are expressed during regeneration. However, in cases where no specific inhibitor is available for the gene product of interest, determination of a ...

Current approaches to tissue regeneration are limited by the death of most transplanted cells and/or resultant poor integration of transplanted cells with host tissue. We hypothesized that transplanting progenitor cells within synthetic microenvironments that maintain viability, prevent terminal differentiation, and promote outward migration would significantly enhance their repopulation and re...

*Address correspondence to: Leonor Santos-Ruiz. Departamento de Biología Celular y Genética, Facultad de Ciencias, Universidad de Málaga, Spain. e-mail: [email protected] ABSTRACT Teleost fins can be structural and functionally restored after severe injuries or partial amputation through a process of epimorphic regeneration, which requires an acute temporal and spatial control of basic cell events...

lemon balm (melissa officinalis l.) is an herbaceous perennial plant of the lamiaceae family that has a wide edible and medicinal uses. in this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on ms mediums supplemented with different concentrations of 6-benzylaminopurine (bap) (2.2, 4.4 and 8.8 µm) alone or in combination wit...

Orthotopic liver transplantation was performed in 60 recipient rats weighing 200 to 250 gm. Sixty rats of the same strain were used as liver donors, 30 weighing 100 to 140 gm (small for size) and the other 30 weighing 200 to 250 gm (same size). After 1, 2, 3, 4, 7 and 14 days (n = 5 each) DNA synthesis, nuclear thymidine labeling and mitoses were increased in both the small-for-size and same-si...

Direct videomicroscopic visualization of organ formation and regeneration in toto is a powerful strategy to study cellular processes that often cannot be replicated in vitro. Intravital imaging aims at quantifying changes in tissue architecture or subcellular organization over time during organ development, regeneration or degeneration. A general feature of this approach is its reliance on the ...

Transit-amplifying cells (TACs) are an early intermediate in tissue regeneration. Here, using hair follicles (HFs) as a paradigm, we show that emerging TACs constitute a signaling center that orchestrates tissue growth. Whereas primed stem cells (SCs) generate TACs, quiescent SCs only proliferate after TACs form and begin expressing Sonic Hedgehog (SHH). TAC generation is independent of autocri...

BACKGROUND General and local anesthetics are used in various combinations during surgical procedures to repair damaged tissues and organs, which in almost all instances involve nervous system functions. Because synaptic transmission recovers rapidly from various inhalation anesthetics, it is generally assumed that their effects on nerve regeneration and synapse formation that precede injury or ...

IMPORTANCE Facial nerve injury leads to severe functional and aesthetic deficits. The transgenic Thy1-GFP rat is a new model for facial nerve injury and reconstruction research that will help improve clinical outcomes through translational facial nerve injury research. OBJECTIVE To determine whether serial in vivo imaging of nerve regeneration in the transgenic rat model is possible, facial n...

the present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (pgrs) combinations under in vitro conditions. the cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in ms medium containing ba (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and tdz (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...