With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for measuring anticancer drugs, but currently few biosensors can achieve this goal. In particular, it is challenging to have a general method to monitor various types of anticancer...

When an adequate amount of an antigenie chemical such as dinitroehlorobenzene (DNCB) is applied to the skin of a guinea pig, an allergic contact sensitivity to that compound may result; this sensitivity is analogous to contact sensitivity in man. After the appearance of sensitization, however, further application of the same chemical to the skin may eventually produce a specific depression of s...

We show that N-acetylcysteine (NAC) has the ability to cause statistically significant diminishment in the in vitro formation of irreversibly sickled cells (ISCs) at concentrations greater than 250 micromol/L. Other antioxidants, approved for human use (cysteamine, succimer, dimercaprol), were not efficacious. NAC had the ability to cause statistically significant conversion of ISCs formed in v...

On bases of previous observations on the band 3 anion exchange inhibition by the electrophilic reagent 1-fluoro-2,4-dinitrobenzene in resealed ghosts and a previously proposed band 3-based mechanism of control of the erythrocyte shape, it is suggested that stomatocytoses by 1-chloro-2,4-dinitrobenzene and other electrophilic reagents are due to an inhibition of the band 3 anion exchange by a co...

The steady-state kinetics of the dimeric glutathione transferases deviate from Michaelis-Menten kinetics, but have hyperbolic binding isotherms for substrates and products of the enzymic reaction. The possibility of subunit interactions during catalysis as an explanation for the rate behaviour was investigated by use of rat isoenzymes composed of subunits 1, 2, 3 and 4, which have distinct subs...

GST (glutathione transferase) is a dimeric enzyme recognized for biotransformation of xenobiotics and endogenous toxic compounds. In the present study, residues forming the hydrophobic substrate-binding site (H-site) of a Delta class enzyme were investigated in detail for the first time by site-directed mutagenesis and crystallographic studies. Enzyme kinetics reveal that Tyr111 indirectly stab...

Nonspecific ¡mmunocompetencein VX2 tumor-bearing rabbits was assessed by skin response to dinitrochlorobenzene and development of antibody titer to bovine serum albumin. Skin reactivity to a 3 M KCI extract of the VX2 and antitumor antibody titer were used to monitor host immunocompetence against the tumor. Rabbits bearing a 15to 20-ml i.m. VX2 tumor in the hind limb were treated by local and ...