Methods currently in use for preparation of cell suspensions for tissue culture are useful for tumor transplantation work and permit quantitative assays of factors affecting transplantation. Enzymatic digestion yields higher numbers of viable cells than the commonly used mechanical method of squeezing cells through metal meshes. Cell suspensions prepared by enzymatic digestion can be stored at ...

Occupied Regions of Genomes from Affinity-purified Naturally Isolated Chromatin (ORGANIC) is a high-resolution method that can be used to quantitatively map protein-DNA interactions with high specificity and sensitivity. This method uses micrococcal nuclease (MNase) digestion of chromatin and low-salt solubilization to preserve protein-DNA complexes, followed by immunoprecipitation and paired-e...

A method is described which allows to reveal simultaneously the proteolytic patterns of numerous polypeptides separated by two-dimensional electrophoresis. After two-dimensional electrophoresis, the gels were dipped successively in buffers for preequilibration, protease digestion, and reequilibration. They were then returned to the electrophoresis tank, and electrophoresis was continued for a s...

An account of previous work on cathepsin may be found in the monograph of Pozzi (1935). In the hemoglobin method for the estimation of protelnase, denatured hemoglobin is digested and the digestion products not precipitable by trichloracefic acid are estimated colorimetrically. Hemoglobin, unlike commercial casein, edestin, and gelatin, is a reproducible substrate, Only the very first stages of...

Although it has become clear that brush-border hydrolysis, and consequent utilization of the monosaccharide transport system, is the predominant method of absorption of the luminal products of carbohydrate digestion in man, it now appears certain that two major mechanisms are involved in the absorption of the luminal products of protein digestion: ( I ) transport of liberated free amino acids b...

Digestion of polyoma viral DNA with a restriction enzyme from Haemophilus aegyptius generates at least 22 unique fragments. The fragments have been characterized with respect to size and physical order on the polyoma genome, and the 5' to 3' orientation of the (+) and (-) strands has been determined. A method for specific radiolabeling of adjacent fragments was employed to establish the fragmen...