Discarded carrots are a major food waste that is produced from cultivation to sale. This has high humidity, which generates not only economic but also environmental problems, requiring specific biorefinery for its valorization. The pulp and the juice were separated received different treatment. was subjected process of separation purification using several diafiltration cycles (30 kDa) giving r...

The current state of the art on studying whey is considered. processes and methods processing (thermal, chemical, physicochemical, biotechnological, electrophysical) are presented. Thermal isoelectric precipitation proteins, using reagents coagulants, as well main membrane (reverse osmosis, diafiltration, microfiltration, ultrafiltration, nanofiltration) described. Possibi-lities effective sepa...

Coxsackievirus A16 (CA16) and enterovirus 71 (EV71), both of which can cause hand, foot and mouth disease (HFMD), are responsible for large epidemics in Asian and Pacific areas. Although inactivated EV71 vaccines have completed testing in phase III clinical trials in Mainland China, CA16 vaccines are still under development. A Vero cell-based inactivated CA16 vaccine was developed by our group....

Hypoallergenic antibacterial low-molecular-mass peptides were produced from defatted soybean meal in a membrane bioreactor. In the first step, soybean meal proteins were digested with trypsin in the bioreactor, operated in batch mode. For the tryptic digestion of soybean meal protein, optimum initial soybean meal concentration of 75 g/L, temperature of 40 °C and pH=9.0 were determined. After en...

Effects of oxidative browning inhibitors on sweet potato protein (SPP) recovery and quality were studied. Oxidative browning inhibitors successfully decreased sweet potato oxidative browning, but reduced both SPP extractability and recovery. Ultrafiltration/diafiltration processed sweet potato (UDSP) protein (at pH 4, 6 and 7) showed significantly (p<0.05) higher yield, purity, solubility, ther...

The association efficiency of oxytetracycline (OTC) to pharmaceutical available, ionic oil-in-water nanoemulsions is studied. Theoretical mathematical developments allowed us to differentiate by diafiltration (DF) between thermodynamically and kinetically controlled binding of the drug to the nanoemulsions, and relate these important magnitudes to the association efficiency. The nanoemulsions h...

Recent advances in generating active proteins through refolding of bacterial inclusion body proteins are summarized in conjunction with a short overview on inclusion body isolation and solubilization procedures. In particular, the pros and cons of well-established robust refolding techniques such as direct dilution as well as less common ones such as diafiltration or chromatographic processes i...

Isolated soy-protein products are useful for food ingredients because they provide solubility, water binding, fat adsorption, emulsification, viscosity, gelation, foaming, adhesion and cohesion, texturization, and flavor formation. Soy-protein concentrates (SPCs) and soy (SPIs) are the representative soy Conventionally, SPCs and SPIs have been produced by removing non-protein portions such as o...

The manufacturing of bispecific antibodies can be challenging for a variety of reasons. For example, protein expression problems, stability issues, or the use of non-standard approaches for manufacturing can result in poor yield or poor facility fit. In this paper, we demonstrate the use of standard antibody platforms for large-scale manufacturing of bispecific IgG1 by controlled Fab-arm exchan...