the stromal-vascular fraction separated from the adipocytes by centrifugation. T h e stromal-vascular cells were seeded ( 2 0 0 0 0 0 cells/ml; 2 m1/25 cm? tissue culture flask) in Medium 199 supplemented with 200h (v/v) fetal calf serum and incubated at 37°C. T h e adipocyte fraction from the digests was also collected and washed before being added ( I ml of cells/25 cm? tissue culture flask) ...

With the increasing clinical translation of adipose stromal cell based technologies, it is important to thoroughly report on all aspects of these technologies. In this article, we review the commonly used enzymes for adipose tissue dissociation and stromal vascular fraction isolation. The enzymes reviewed include collagenase, trypsin, clostripain, and the neutral proteases dispase and thermolys...

We present a series of ten patients with non-reconstructable peripheral vascular disease (PVD), secondary to arteriosclerosis (AS) and/or diabetes mellitus (DM), treated with local injection of non-expanded autologous, adipose-derived stromal vascular fraction (SVF) cells for the purposes of enhancing neovascularization and chronic wound healing. Adipose tissue was surgically harvested and proc...

Every year millions of Americans are affected by cardiovascular and peripheral arterial diseases. When a patient’s own blood vessels are not suitable for surgical intervention, alternative graft sources are necessary. In this study, the lumens of expanded polytetrafluoroethylene (ePTFE) conduits were coated with freshly isolated adipose-derived stromal vascular fraction (SVF) cells or cultured ...