Biosynthesis of ent-kaurene was investigated in extracts of cell suspension cultures derived from tobacco callus (Nicotiana tabacum L.), tomato callus (Solanum lycopersicum L.), and in germinating tomato seeds. Incubation of extracts derived from the two cell cultures with either isopentenyl pyrophosphate-(14)C or with (14)C-labeled mevalonate, followed by alkaline phosphatase hydrolysis, resul...

In this study the effect of different concentrations of some abiotic elicitors (salicylic acid, jasmonic acid, cadmium chloride and sodium chloride) with different incubation times (3, 6, 9 and 12) on azadiractin production and cell growth in the cell culture of the Iranian native Neem were investigated. Cell growth rate was measured by changes in dry cell weight and it was used as a basis for ...

developing seeds of oilseed rape is fundamental to the rational development of biotechnological strategies for The effect of sucrose concentration on triacylglycerol increasing seed oil content and manipulating oil composibiosynthesis and associated gene expression was tion. In oilseeds, the biosynthesis of TAG in developing examined in a microspore-derived cell suspension culseeds is catalysed...

Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using culture conditions that allow moderate cell proliferation. In view of true-to-type large-scale propagat...

OBJECTIVE Growth factors demonstrate mixed results improving wound healing. Amnion-derived multipotent cells release physiologic levels of growth factors and tissue inhibitors of metalloproteinases. This solution was tested in models of acute and chronic wound healing. METHODS Acute model: Sprague-Dawley rats underwent laparotomy incisions. The midline fascia was primed with phosphate-buffere...

The first two authors contributed equally to this manuscript. Address correspondence to: Maggi Fabrizio, M.D., Ph.D., Virology Unit, Azienda Ospedaliera Universitaria Pisana, Via San Zeno, 35-37, 56127 Pisa, Italy. E-mail: [email protected] Received 28 January 2008. Revision requested 14 February 2008. Accepted 7 March 2008. Copyright © 2008 by Lippincott Williams & Wilkins ISSN 0041-1337/...

Background: Microparticles (MPs) are small vesicles released from the cell membrane. Accordingly, these contain active molecules to mediate biological processes, including cell proliferation and cell cycle progression. The fusion of myeloma cell lines with immunized B lymphocytes is a critical step of hybridoma technology. MPs modulate lymphoproliferation, thereby facilitating B cell expansion ...

background: as condition and component of culture determine fate map of spermatogonial stem cells (sscs), the aim of this study was to evaluate of growth factors gdnf, lif and ra on proliferation and differentiation of ssc. materials and methods: sscs were cultured in two groups: the first group gdnf and lif and the second group ra. the number of clumps and colony formation was monitored during...

Abstract Cell injection is a common clinical approach for therapeutic delivery into diseased and damaged tissues in order to achieve regeneration. However, cell retention, viability, engraftment at the site have generally been poor, driving need improved approaches. Here, we developed technique shrink-wrap micropatterned islands of corneal endothelial cells basement membrane-like layer extracel...