The yeast nucleic acid used in these experiments was a commercial preparation purified by treatment with picric acid (3). A sample of yeast nucleic acid and one of thymus nucleic acid were kindly supplied by Dr. P. A. Levene to whom the author wishes to extend his heartiest thanks. The effect of the enzyme preparations on nucleic acid was followed by determining the amount of total phosphorus r...

A trace lead ion (Pb 2+ ) detection platform combining surface-enhanced Raman spectroscopy (SERS) with nucleic acid cycle multiple signal amplification.

Nucleic acid nanostructures are versatile and nearly infinitely customizable, which can make it challenging to find the right one.

BACKGROUND Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. However, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. Oligonucleotides co...

Until the 1980s the diagnosis of specific etiologic agents of infectious diseases rested with their isolation in vitro and identification by analysis of their phenotypic characteristics. In the 1970s the concept of a microbial species evolved from phenotypic analysis to nucleic acid homology. Currently, nucleic acid sequences specific for a given species are being isolated and amplified and uti...

bstract—Most gap junctions between neurons in mammaian retina contain abundant connexin36, often in association ith the scaffolding protein zonula occludens-1. We now nvestigate co-association of connexin36, zonula occludens-1, onula occludens-2 and Y-box transcription factor 3 (zonula ccludens-1-associated nucleic acid-binding protein) in ouse and rat retina. By immunoblotting, zonula occluden...

chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection, with an estimated annual worldwide incidence of 50 million cases. a large proportion of those infected, particularly women, are asymptomatic, and these individuals serve as a major reservoir of infection. women are also at risk for serious reproductive tract complications with significant morbidity. in an effo...

there are significant challenges associated with qualitative and quantitative nucleic acid tests performed in diagnostic laboratories. the development of internationally available certified reference materials which can be traced to reference measurements will contribute to a better understanding of the performance characteristics of nucleic acid tests and enhance reliability and comparability ...

We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below its melting temperature (T(m)). The difference between these two spectra is the ...