Peptide hormones and neuropeptides are packaged and stored in a specialized intracellular organelle called the dense core vesicle. It remains elusive how peptide cargoes are correctly sorted. In the present study, we show that a highly conserved Golgi-localized protein named HID-1 acts to prevent mis-sorting of peptide cargoes to lysosomes for degradation via a PtdIns3P-dependent trafficking pa...

With the availability of large entity-relationship graphs, finding the best relationship between entities is a problem that has attracted a lot of attention. Given two or more entities, the goal of most algorithms is to produce a graph structure of varying complexity (i.e., a simple path, a minimal weighted tree, or a dense subgraph etc.) as a way of characterizing the relationship between give...

Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles ...

The subnucleolar structure that is involved in rDNA transcription has been controversial. A report by Koberna et al. (2002)(this issue, page 743) adds significant weight toward the idea that dense fibrillar components (DFCs)**Abbreviations used in this paper: DFC, dense fibrillar component; FC, fibrillar center; GC, granular component; Pol I, polymerase I. and fibrillar center (FC)/DFC borders ...

This paper introduces a new definition of dense subgraph pattern, the DN -graph. DN -graph considers both the size of the substructure and the minimum level of interactions between any pair of the vertices. The mining of DN -graphs inherits the difficulty of finding clique, the fully-connected subgraphs. We thus opt for approximately locating the DN -graphs using the state-of-the-art graph tria...

Data sources representing attribute information in combination with network information are widely available in today’s applications. To realize the full potential for knowledge extraction, mining techniques like clustering should consider both information types simultaneously. Recent clustering approaches combine subspace clustering with dense subgraph mining to identify groups of objects that...

Mining dense subgraphs on multi-layer graphs is aninteresting problem, which has witnessed lots of applications inpractice. To overcome the limitations of the quasi-clique-basedapproach, we propose d-coherent core (d-CC), a new notionof dense subgraph on multi-layer graphs, which has severalelegant properties. We formalize the diversified coherent coresearch (DCCS) probl...

Pancreatic β-cells use Ca(2+)-dependent exocytosis of large dense core vesicles to release insulin. Exocytosis in β-cells has been studied biochemically, biophysically and optically. We have previously developed a biophysical method to monitor release of endogenous intragranular constituents that are co-released with insulin. This technique involves the expression of ionotropic membrane recepto...

Rnd proteins are atypical members of the Rho GTPase family that induce actin cytoskeletal reorganization and cell rounding. Rnd proteins have been reported to bind to the intracellular domain of several plexin receptors, but whether plexins contribute to the Rnd-induced rounding response is not known. Here we show that Rnd3 interacts preferentially with plexin-B2 of the three plexin-B proteins,...

The assessment of relationships between satellite-derived vegetation indices and meteorological drought improves our understanding of how these indices respond to climatic changes. The combination of climate data and the Normalized Difference Vegetation Index (NDVI) product of Moderate Resolution Imaging Spectroradiometer (MODIS) imagery provided an opportunity to evaluate the impact of d...