background & objectives: antisense peptide nucleic acids (pna) that target growth essential genes show potent bactericidal properties without cell lysis. we considered the possibility that whether pna treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to dendritic cells (dcs). dcs are the most potent antigen presenting cells in t...

objective: traditional methods for storing microbial dna samples use the low-temperatures of 4 °c, -20°c or -80 °c. the dbc card is a new method of storing microbial dna at room temperature. this study evaluates the stability and determine the best storing method of bacterial dna on the dbc card. methods: in this study, we used a pair of primers from the conserved domain of 16srrna designed to...

the aim of the present study was the conducting of comparative analysis of allele pool of ukrainian population of thoroughbred horses and the populations from england, usa, russia and south korea using microsatellite loci of dna on the basis of our own researches and literary sources. comparative analysis of allele pool of thoroughbred populations from different countries was conducted using 6 ...

DNA origami is a cutting-edge self-assembly technique that neatly folds strands and creates specific structures based on the complementary base pairing principle. These innovative nanostructures provide numerous benefits, including lower biotoxicity, increased stability, superior adaptability, making them an excellent choice for transporting anti-tumor agents. Furthermore, they can considerably...

Nucleic acids, DNA and RNA, not only allow transfer and replication of densely coded genetic information, but also act as danger signals triggering innate immune response. Recent progress in the design and construction of nano-sized structures using DNA has opened a new field of nanotechnology. The unique properties of nano-sized DNA constructs can be exploited to develop programmable materials...

In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a comp...

Vanessa Bastos Pereira; Marcela Santiago Pacheco Azevedo; Tessália Diniz Luerce Saraiva; Bianca Mendes Souza; Meritxell Zurita Turk; Camila Prósperi De Castro; Pamela Mancha Agresti; Fernanda Alvarenga Lima; Vasco Azevedo; Daniela Santos Pontes and Anderson Miyoshi Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Ho...

background: dendritic cells (dcs) are ideal accessory cells in the field of gene therapy. delivery of dna and sirna into mammalian cells is a useful technique in treating various diseases caused by single gene defects. selective gene silencing by small interfering rnas (sirnas) and antisense oligodeoxynucleotides (odn)s is an efficient method for the manipulation of cellular functions. an effic...

DNA vaccines against cancer have shown promising results in animal models. Efficient antigen-specific T cell responses and tumor protection have been demonstrated after vaccination with DNA encoding tumor antigens in mice. Phase I clinical trials have furthermore demonstrated that repetitive DNA vaccinations in humans are well tolerated, and that tumor antigen-specific immune responses can be i...