In earlier studies on the binding of indole analogues with defatted human serum albumin (l), the intrinsic association constants for both a neutral ligand and a ligand bearing a negative charge were found to be invariant in the pH region of 5 to 10, implying that the structure of the binding site on the albumin did not change in this region. As the pH increased or decreased from this region the...

I studied the effects of serum dilution and oxidation of albumin-bound bilirubin on the accuracy of results by the peroxidase method for assessing bilirubin-albumin binding. The apparent concentration of unbound bilirubin decreases with dilution of bilirubin-enriched serum or defatted-albumin solutions, the effect being more marked with serum. The decrease in apparent unbound bilirubin does not...

Fourier transform infrared (FT-IR) spectra have been measured for defatted bovine serum albumin (BSA) in D(2)O with a concentration of 2.0 wt % over a temperature range of 26-90 degrees C and the corresponding difference spectra have been calculated by subtracting the contribution of D(2)O at the same temperature. Evolving factor analysis (EFA) by selecting two factors and three factors has bee...

In this work, simultaneous isolation of lactoferrin (Lf) and lactoperoxidase (Lp) from defatted bovine colostrum by one-step cation exchange chromatography with SPEC 70 SLS ion-exchange resin was investigated. A RP-HPLC method for Lf and Lp determination was developed and optimized as the following conditions: detection wavelength of 220 nm, flow rate of 1 mL/min and acetonitrile concentration ...

BACKGROUND The cholesterol-lowering abilities of rice bran's fiber and oil apart from its fatty acid composition remain unclear. OBJECTIVE The objective of the study was to assess the effects of defatted rice bran and rice bran oil in an average American diet on blood lipids in moderately hypercholesterolemic persons. DESIGN Study 1 used a parallel-arm design. Twenty-six healthy volunteers ...

Screen analysis Table 1-Screen analyses of sorghum and soy grits, reground products and cottonseed flour 1969) from yellow sorghum with less than 2% of kernels having brown subcoats. Brown-seeded sorghums were minimized because they have lower protein nutritional value and digestibility (Axtell et al., 1975) and contain pigments which can darken the fmal product as shown by Rooney et al. (1970)...

aerial parts of salvia mirzayanii was extracted with methanol. methanol extract was suspended in water and defatted with petroleum ether. the defatted part was then partitioned between ethyl acetate and water. the ethyl acetate partition was chromatographed on a silica gel column to afford several fractions. lymphocyte proliferation inhibitory assay of the resulted fractions was compared in vit...

Flaxseed, the richest source of mammalian lignan precursors, such as secoisolariciresinol diglycoside (SD), has been shown over the short term to decrease some early markers of colon cancer risk. This study determined whether over the long term flaxseed still exerts a colon cancer protective effect, whether its effect may, in part, be due to its high content of SD and whether any change in beta...

In earlier studies on the binding of indole analogues with defatted human serum albumin (l), the intrinsic association constants for both a neutral ligand and a ligand bearing a negative charge were found to be invariant in the pH region of 5 to 10, implying that the structure of the binding site on the albumin did not change in this region. As the pH increased or decreased from this region the...