In this study, two thermal-tolerant mutants of Chlorella sp. MT-7 and MT-15, were isolated. In indoor cultivation, specific growth rate (micro, d(-1)) of the mutants were 1.4 to 1.8-fold at 25 degrees Celsius and 3.3 to 6.7-fold at 40 degrees Celsius higher than those of wild type. The carbon dioxide fixation rate of both microalgal mutants was also significantly higher than that of wild type. ...

Extensive expansion of mesenchymal stem cells (MSCs) for cell-based therapies remains challenging since long-term cultivation and excessive passaging in two-dimensional conditions result in a loss of essential stem cell properties. Indeed, low survival rate of cells, alteration of surface marker profiles, and reduced differentiation capacity are observed after in vitro expansion and reduce ther...

Genetically modified (GM) maize expressing the insecticidal protein Cry1Ab from Bacillus thuringiensis (Bt maize) is the only GM crop planted commercially in the European Union (EU). Cultivation in accordance with Directive 2001/18/EC demands post-market environmental monitoring (PMEM) to ensure the detection and prevention of adverse effects on the environment possibly deriving from commercial...

Cells must coordinate adjustments in genome expression to accommodate changes in their environment. We hypothesized that the amount of transcriptome change is proportional to the amount of environmental change. To capture the effects of environmental changes on the transcriptome, we compared transcriptome diversities (defined as the Shannon entropy of frequency distribution) of silkworm fat-bod...

The application of high-throughput sequencing of the 16S rRNA gene has increased the size of microbial diversity datasets by several orders of magnitude, providing improved access to the rare biosphere compared with cultivation-based approaches and more established cultivation-independent techniques. By contrast, cultivation-based approaches allow the retrieval of both common and uncommon bacte...

lack of sewage collection systems, percolation of surface waters, and seepage of wells have raised the groundwater table in shiraz area in the south of iran. the growing population generates environmental pollution resulting in the degradation of the quality of surface and groundwaters used for agriculture. inorganic and organic pollutants have been traced in shiraz water resources. heavy metal...

background : leishmaniasis is one of the infectious parasitic diseases of highest incidence in the world. cutaneous leishmaniasis (cl) has long been reported in shiraz, southern iran. there is a need to find a sensitive and specific method for treatment and control of the disease. methods: we have compared the sensitivity of the conventional methods microscopy and cultiva­tion of lesion scrapes...

Sponges host complex microbial communities of recognized ecological and biotechnological importance. Extensive cultivation efforts have been made to isolate sponge bacteria, but most still elude cultivation. To identify the bottlenecks of sponge bacterial cultivation, we combined high-throughput 16S rRNA gene sequencing with a variety of cultivation media and incubation conditions. We aimed to ...

Myxobacteria harbor an enormous potential for new bioactive secondary metabolites and therefore the isolation of in particular new groups is of great interest. The diversity of myxobacteria present in two ecological habitats, namely sand from Kiritimati Island and German compost, was evaluated by both cultivation-based and cultivation-independent methods. Phylogenetic analyses of the strains in...

23 The nodulation tendency and community structure of indigenous bradyrhizobia on 24 Rj-genotype soybean cultivars at cultivation temperatures of 33/28°C, 28/23°C and 23/18°C, 25 16/8 h (day/night degrees, hours) were investigated using 780 bradyrhizobial DNA samples 26 from an Andosol with 13 soybean cultivars of four Rj-genotypes (non-Rj, Rj2Rj3, Rj4 and 27 Rj2Rj3Rj4). A dendrogram was constr...