Animal-derived xenogeneic biomaterials utilized in different surgeries are promising for various applications tissue engineering. However, decellularization is necessary to attain a bioactive extracellular matrix (ECM) that can be safely transplanted. The main objective of the present study assess structural integrity, biocompatibility, and potential use acellular engineering applications. Henc...

End-stage organ failure is a major global issue, with the liver being second-highest transplanted due to lifestyle choices or other conditions. In field of biomedical engineering, artificial manufacturing has been possible alternative transplants by aiming achieve less immune rejection, more efficient production, and higher accessibility. Of biological methods today, two up-and-coming technolog...

BACKGROUND The xenoantigenicity of porcine bioprosthetic valves is implicated as an etiology leading to calcification and subsequent valve failure. Decellularization of porcine valves theoretically could erase the antigenicity of the tissue leading to more durable prosthetic valves, but the effectiveness of decellularization protocols in regard to completely removing antigens has yet to be veri...

results migration of cells started on the fifth day. as a result of this migration, most cells were located on the surface of the scaffold and formed epiderm-like structure and some penetrated into the scaffold. by day 25, cells which had penetrated into the scaffold had various destinies such as becoming elongated spindle-shaped cells and creating blood vessel-like structures. conclusions adm ...

Perfusion-based whole organ decellularization has recently gained interest in the field of tissue engineering as a means to create site-specific extracellular matrix scaffolds, while largely preserving the native architecture of the scaffold. To date, this approach has been utilized in a variety of organ systems, including the heart, lung, and liver (1-5). Previous decellularization methods for...

The clinical use of decellularized cardiac valve allografts is increasing. Long-term data will be required to determine whether they outperform conventional cryopreserved allografts. Valves decellularized using different processes may show varied long-term outcomes. It is therefore important to understand the effects of specific decellularization technologies on the characteristics of donor hea...

One of the main challenges currently faced by tissue engineers is the loss of tissues postimplantation due to delayed neovascularization. Several strategies are under investigation to create vascularized tissue, but none have yet overcome this problem. In this study, we produced a decellularized natural vascular scaffold from rat intestine to use as an in vitro platform for neovascularization s...

The surgical reconstruction of ureteric defects is often associated with post-operative complications and requires additional medical care. Decellularized ureters originating from porcine donors could represent an alternative therapy. Our aim was to investigate the possibility of manufacturing decellularized ureters, the characteristics of the extracellular matrix (ECM) and the biocompatibility...

PURPOSE Tectonic lamellar keratoplasty (TLKP) is a primary surgical procedure to improve the condition of the recipient bed in high-risk corneal transplantation. It is usually performed for a secondary optical penetrating keratoplasty (PKP). The present study was undertaken to explore a new strategy for TLKP using acellular corneal stroma (ACS) prepared by decellularization. METHODS ACS for T...