In the present study, we determined the involvement of brainstem catecholaminergic inputs to the paraventricular nucleus (PVN) on estrogen receptor alpha (ERalpha) expression in this nucleus during conditions of 48-h fasting, 2-deoxy-d-glucose (2DG)-induced acute glucoprivation and 1-h immobilization, in ovariectomized rats. Our approach was to examine the effect of lesioning catecholaminergic ...

The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), enhances transcription of many eukaryotic genes, including that for dopamine b-hydroxylase (DBH). In the present study, we report identification and characterization of a novel sequence motif residing in the 5*-flanking region of the human DBH gene, which mediates transcriptional induction by TPA. Deletional analyses indicated the p...

The resistance of breast cancer to radiotherapy remains a major obstacle to successful cancer management. Radiotherapy may result in DNA damage and activate breast cancer stem cells. DNA damage may lead to activation of the checkpoint kinase (CHK) signaling pathway, of which debromohymenialdisine (DBH) is a specific inhibitor. Radiotherapy also increases the expression of phosphorylated CHK1/2 ...

In viva biosynthesis of the 2 subunit forms of dopamine B-hydroxylase (DBH) was examined in the rat brain. %-methionine was injected into the noradrenergic neurons of the locus coeruleus (LC) using a stereotactic device. Several hours later, newly synthesized 35S-Met-labeled DBH was immunoprecipitated and quantitated. Both M, = 77,000 (77K) and 73,000 (73K) subunit forms were present in near-eq...

Featured Article: Nagatsu T and Udenfriend S. Photometric assay of dopamine-hydroxylase in human blood. Clin Chem 1972;18:980 –3. This report described a simple assay for measuring the activity of dopamine-hydroxylase (DBH) in human blood. DBH is a copper-containing, ascorbaterequiring glycoprotein enzyme that catalyzes the hydroxylation of dopamine to norepinephrine (NE), the precursor of epin...

This experiment assessed the yield and growth performance of the fodder tree planting in the community forest focusing on Artocarpus lakoocha, Bahunia purpurea. Major variables analyzing the yield and growth of the species such as dbh, height, green weight of the foliage was measured. After analyzing the data, Artocarpus lakoocha was found to be good yielding and growth performing species as co...

We assessed the effect that varying patterns of biomass allocation had on growth of ponderosa pine (Pinus ponderosa) growing in the desert climate of the Great Basin and the montane climate of the eastern Sierra Nevada. Prior work established that desert trees have lower leaf: sapwood area ratios than montane trees (0.104 and 0.201 M2/ cm2, respectively) and proportionally greater stem respirat...

0378-1127/$ see front matter 2012 Elsevier B.V. A http://dx.doi.org/10.1016/j.foreco.2012.08.004 ⇑ Corresponding author. Tel.: +1 612 626 4280; fax E-mail addresses: [email protected] (M.B. Russell) (A.R. Weiskittel). 1 This research was conducted while M.B. Russell w School of Forest Resources. Snags are an important component of forest structure and a key carbon pool, but there is currently a ...

We have investigated the specification of noradrenergic neurotransmitter identity in neural crest stem cells (NCSCs). Retroviral expression of both wild-type and dominant-negative forms of the paired homeodomain transcription factor Phox2a indicates a crucial and direct role for this protein (and/or the closely related Phox2b) in the regulation of endogenous tyrosine hydroxylase (TH) and dopami...