Galactose mutarotase catalyzes the conversion of beta-d-galactose to alpha-d-galactose during normal galactose metabolism. The enzyme has been isolated from bacteria, plants, and animals and is present in the cytoplasm of most cells. Here we report the x-ray crystallographic analysis of human galactose mutarotase both in the apoform and complexed with its substrate, beta-d-galactose. The polype...

The first committed step in the biosynthesis of L-ascorbate from D-glucose in plants requires conversion of GDP-L-galactose to L-galactose 1-phosphate by a previously unidentified enzyme. Here we show that the protein encoded by VTC2, a gene mutated in vitamin C-deficient Arabidopsis thaliana strains, is a member of the GalT/Apa1 branch of the histidine triad protein superfamily that catalyzes ...

A highly stereoselective, efficient and facile route was achieved for the synthesis of novel biochemically potent sugar fused pyrano[3,2- c ]pyranone derivatives starting from inexpensive, naturally occurring d -galactose -glucose.

Reactive oxygen species (ROS) are closely related to the aging process. In our previous studies, we found that the saponins from Aralia taibaiensis have potent antioxidant activity, suggesting the potential protective activity on the aging. However, the protective effect of the saponins and the possible underlying molecular mechanism remain unknown. In the present study, we employed a D-galacto...

The metabolic network in E. coli can be severely affected by the inactivation of metabolic genes that are required to catabolize a nutrient (D-galactose). We hypothesized that the resulting accumulation of small molecules can yield local as well as systemic effects on the metabolic network. Analysis of metabolomics data in wild-type and D-galactose non-utilizing mutants, galT, galU and galE, re...

Aluminum (Al) accumulation increases with aging, and long-term exposure to Al is regarded as a risk factor for Alzheimer's disease. In this study, we investigated the effects of Al and/or D-galactose on neural stem cells, proliferating cells, differentiating neuroblasts, and mature neurons in the hippocampal dentate gyrus. AlCl3 (40 mg/kg/day) was intraperitoneally administered to C57BL/6J mice...

Aging is the process spontaneously occurred in living organisms. Cardiac fibrosis a pathophysiological of cardiac aging. Mangiferin well-known C-glucoside xanthone mango leaves with lots beneficial properties. In this study, rat model was induced by injected 150 mg/kg/d D-galactose for 8 weeks. The age-related decline estimated detecting relative weight heart, serum levels injury indicators and...

KIT and / or PDGFRA activation mutation is the initial molecular event of gastrointestinal stromal tumor (GIST), mechanism leading to malignant progression GIST needs be further studied broken through. This study aims understand from perspective metabolic reprogramming provide a new target for treatment GIST. In present study, transcriptome metabolome were used test tissue adjacent normal sampl...