background: human cd34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (hscs) and the small- vessel endothelial cells of a variety of tissues. cd34 plays a critical role as a marker for diagnosis and classification of leukemia. anti cd34 antibodies are used for isolation and purification of hscs from bone marrow, peripheral blood and cord blood. objective: t...

a multi‑parameter quantification method was implemented to quantify retinal vascular injuries in microscopic images of clinically relevant eye diseases. this method was applied to wholemount retinal trypsin digest images of diabetic akita/+, and bcl‑2 knocked out mice models. five unique features of retinal vasculature were extracted to monitor early structural changes and retinopathy, as well ...

• Introduction: Microbial Flow Cytometry: Technology and Applications. Martin G. Wilkinson • Chapter 1: Microbial Cytometry: What It Was, Is, and May Be. Howard M. Shapiro • Chapter 2: Non-Destructive OnChip Imaging Cytometry Assay for Constructive On-Chip Cellomics Studies. Kenji Yasuda • Chapter 3: Application of Flow Cytometry to Environmental Biotechnology. Mónica Herrero and Mario Díaz • C...

BACKGROUND Elasticity of cells is determined by their cytoskeleton. Changes in cellular function are reflected in the amount of cytoskeletal proteins and their associated networks. Drastic examples are diseases such as cancer, in which the altered cytoskeleton is even diagnostic. This connection between cellular function and cytoskeletal mechanical properties suggests using the deformability of...

Oral presentations (alphabetical order) Interferon-gbeeinflußt den Apoptose-Signalweg von TNFa nur teilweise über NFkB in der humanen Tumor Zell-Linie Me-180 Heinz BAISCH Institut für Biophysik und Strahlenbiology, Universitäts Klinikum Hamburg-Eppendorf In Me-180 Zellen induziert TNFa Apoptosis. Die Zellen lösen sich vom Kulturflaschenboden und zeigen apoptotische Charakteristika wie Chromatin...

FLOW CYTOGENETICS Flow cytometric analyses of individual chromosomes were made possible by the development of techniques for isolating intact chromosomes (1) and by procedures for staining chromosomes with DNA-specific fluorescent dyes. The term flow cytogenetics (2) was coined in the early 1980s to describe the application of flow cytometry and sorting to chromosome classification and purifica...

State-of-the-art high-throughput microscopes are now capable of recording image data at a phenomenal rate, imaging entire microscope slides in minutes. In this paper we investigate how a large image set can be used to perform automated cell classification and denoising. To this end, we acquire an image library consisting of over one quarter-million white blood cell (WBC) nuclei together with CD...

background: flow cytometry is a rapid, sensitive, and reliable method for determination of bacterial viability. here we assayed the capability of flow cytometry to detect helicobacter pylori viable cells in both forms of spiral and coccoid. methods: viable bacteria stained with rhodamin 123 and fluoresced with laser beam of 488nm. the rate of rh123 absorption was determined in both forms of bac...