Cyclic alkyl(amino) carbene-anchored monoanionic phosphorus species have been introduced as the rising ligand to stabilize three unprecedented homo (AgI)- and mixed (Ag0/AgI)-valence silver nanoclusters Ag29, Ag12 Ag8 in solid state with defined structures. The neutral Ag29 clusters showed electron paramagnetic resonance at room temperature, whereas green-emitting tri-cationic cluster is diamag...

One of the mechanisms that minimize the aberrant cross-talk between cAMP- and cGMP-dependent signaling pathways relies on the selectivity of cAMP binding domains (CBDs). For instance, the CBDs of two critical eukaryotic cAMP receptors, i.e. protein kinase A (PKA) and the exchange protein activated by cAMP (EPAC), are both selectively activated by cAMP. However, the mechanisms underlying their c...

A series of [Re(5-R-phen)(CO)3(X)]0/+, where phen = 1,10-phenanthroline, R H, CN, COOH, COONH2, and X Cl or pyridine, was prepared characterized by elemental analyses, NMR, Vis/UV, emission cyclic voltammetry. The complexes absorb in the near-ultraviolet region spectrum, emit between 550 600 nm with lifetimes 150 500 ns, undergo irreversible oxidation at 1.45 V a reversible reduction 1,10-phena...

Cyclic nucleotide-gated ion channels of retinal photoreceptors and olfactory neurons are differentially activated by ligands that vary only in their purine ring structure. The nucleotide selectivity of the bovine rod cyclic nucleotide-gated channel (cGMP > cIMP >> cAMP) was significantly altered by neutralization of a single aspartic acid residue in the binding domain (cGMP > or = cAMP > cIMP)....

A combined ligand and structure-based drug design approach provides a synergistic advantage over either methods performed individually. Present work bestows a good assembly of ligand and structure-based pharmacophore generation concept. Ligand-oriented study was accomplished by employing the HypoGen module of Catalyst in which we have translated the experimental findings into 3-D pharmacophore ...

In developing Dictyostelium discoideum cells, binding of cyclic AMP to the chemotactic receptor has been shown to oscillate. These oscillations represent cycles of activation, adaptation and deadaptation of the cyclic AMP receptor system. We studied the molecular basis of these oscillatory changes in cyclic AMP receptor binding. We developed a rapid method of lysing cells during the course of t...