The genomes of two Ralstonia pickettii strains (H2Cu2 and H2Cu5), isolated from hospital effluent in a selective medium containing CuSO4, were sequenced. They presented MICs of >256 and 6 µg/ml for the aminoglycoside gentamicin, respectively. The 5.2-Mb draft genomes have 40 contigs for strain H2Cu2 and 113 for H2Cu5.

In Saccharomyces cerevisiae, at least 12 genes are important for cells to propagate in medium containing elevated concentrations of copper salts (J. Welch, S. Fogel, C. Buchman, and M. Karin, EMBO J. 8:255-260, 1989). Complementation studies were carried out on a copper-sensitive mutation (cup14) from this group. A new yeast gene, designated SLF1, was identified as a multicopy suppressor of the...

High affinity IgG autoantibodies (ABs) against oxLDLs and lag-phase of serum oxidation were tested in patients with coronary heart disease (CHD). Fifty one (37 M/14 F) patients with CHD defined as Q-wave myocardial infarction and/or stenosis of more than 50% and 51 (34 M/17 F) healthy blood donors as controls participated in this study. LDLs were isolated by gradient ultracentrifugation and oxi...

We investigated the effects of Cu2+-loaded silicate (CLS) on the growth performance, microflora of skin, gill and intestine, and intestinal morphology of crucian carp Carassius auratus. A total of 225 native wild crucian carp, with an average initial body weight of 20 g, were randomly divided into 5 treatment groups using 3 replicate tanks of 15 fish per group. The dietary treatments were (1) b...

Copper nanoparticles (CuNPs) have been widely used in various industrial and commercial applications, which become a potential threat to aquatic organisms. Nevertheless, their toxicity the epididymis sperm remains little known. In this study, we evaluated effect of CuNPs copper ions (CuSO4) on spermatozoa viability, epididymal structure, antioxidant enzyme activity, inflammatory cytokines cauda...

Selective oxidation is still a challenge for targeted conversion of biomass derived substance to high-value added products. Herein we report an efficient electrochemical strategy transform phenol into p-benzoquinone, by tandem oxidations CuSO4 and CoSO4 catalysts in electrolyte. Co3+ generated at the anode from Co2+ diffuses solution reacts with form hydroquinone, which subsequently oxidized Cu...

A ligand exchange HPLC method for enantioseparation of ofloxacin enantiomers was developed by a using C8 stationary phase. Chromatographic separation was performed on a Zorbax-300SB-C8 column eluted with the mobile phase consisting of 15 % methanol in water containing 10mM phenylalanin and 5 mM CuSO4 at flow rate of 0.8 mL/min. The method was simple, rapid and suitable for routine analytical st...

In this work the physiological effect of copper excess on the peach rootstock MrS 2/5 and GF 677 was studied by means of an in vitro system. Two different copper treatments (10 and 100 μM CuSO4) for each rootstock were carried out and compared to a control containing 0.1 μM of CuSO4. Relative growth rate and copper concentration inside rootstock tissues were measured after 21 days from the begi...