نتایج جستجو برای: culture substrates

تعداد نتایج: 353553  

Journal: :Advanced materials 2014
Changyong Cao Hon Fai Chan Jianfeng Zang Kam W Leong Xuanhe Zhao

A simple method for fabricating high-aspect-ratio, hierarchical, and dynamically tunable surface patterns is invented by harnessing localized-ridge instabilities in gold nanofilms coated on elastomer substrates (a); a theoretical model to calculate the critical parameters (e.g., wavelength and amplitude) for designing the new patterns is developed (b); and novel applications of the patterns as ...

2004
Adriane Bianchi Pedroni Medeiros Pierre Christen Sevastianos Roussos Juliana Carine Gern Carlos Ricardo Soccol

The ability of two different strains of Ceratocystis fimbriata for fruity aroma production by solid state fermentation (SSF) was tested on coffee pulp and coffee husk complemented with glucose as substrates. Experiments were carried out in 250 mL Erlenmeyer flasks and the experimental conditions were: 70% of initial moisture, 20% of glucose addition and pH 6.0. Aeration was made by passive diff...

2014

Mammalian cell culture has served as an invaluable tool in cell biology for several decades. Mono­ layers of adherent cells grown on flat and rigid two-dimensional (2D) substrates, such as polysty­ rene or glass, have evolved as the mainstay in conventional cell culture systems. Two-dimensional cell culture studies have played a pivotal role in furthering our understanding of developmental biol...

2006
Fanny Evenou Teruo Fujii Yasuyuki Sakai

Current in vitro cell culture technologies present some limitations as they can't simulate or mimic in vivo situations. Indeed, in vivo cells function in a three-dimensional (3D) structure where they have a close contact with adjacent cells. In this study, human hepatocarcinoma Hep G2 cells were cultured on 3D micropatterned polydimethylsiloxane (PDMS) substrates. Using soft-lithography techniq...

2017
Noel L. Shaheen Esha Kataria Jocelyn Antony Dana Galvan Yessenia Ballou Brad A. Bryan

Angiosarcoma is a rare and generally fatal tumor composed of aberrant cells of endothelial origin. Because of its infrequency in humans, very little is known about the growth requirements of this vascular sarcoma. Unlike the rapidly proliferating solid tumors from which they are isolated from, many of the established angiosarcoma cell lines exhibit less than robust growth in culture and often f...

Biodegradation of phenol in the presence of glucose as a supplementary substrate was investigated with mixed microbial consortium isolated from waste effluent of coke-steel factory. Batch experiments were carried out at room temperature and pH value of 7. Initial phenol and glucose concentrations were in the range of 25-1000 mg/l and 500-3000 mg/l, respectively. In a dual substrates system the ...

Journal: :Biotechnology and bioengineering 1989
J Varani S E Fligiel D R Inman D L Helmreich M J Bendelow W Hillegas

Two human squamous carcinoma cell lines and human diploid fibroblasts were examined for the production of extracellular matrix (ECM) molecules including fibronectin (FN), laminin (LN), and thrombospondin (TSP) when grown on a number of different substrates. The substrates used included glass, plastic, collagen (gelatin), and DEAE-dextran. Levels of TSP as indicated by enzyme-linked immunosorben...

Journal: :international journal of advanced biological and biomedical research 2014
parviz rahbarian ali salehi sardoei

an important factor in the growth medium of plants and chemical plants. native substrates can be used to improve plant performance. to evaluate the effect of growth medium on the herb f. benjamina experimental design was completely randomized with eight treatments and four replications were carried out in the research greenhouse of islamic azad university of jiroft. f. benjamina  plants from ve...

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