Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in efficient manufacturing. We are exploring the use of rapid “holistic” analytical methods that can be used for routine screening of cell culture media used in industrial biotechno...

The green colonial microalga, Botryococcus braunii is well known for its high lipid content and has already been proposed as a renewable energy source for various aquaculture and biotechnological applications. However, due to its slow growth rate compared with other microalgae, B. braunii has not yet been used in mass culture to produce more biomass. Therefore, in this study we tested different...

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the g...

Microbial cells are producers of natural polymers present in plant cells. Production of pullulan (an extracellular microbial polysaccharide) by Aureobasidium pullularia pullulans (P. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. Pullulan formation obeyed a growth and non-growth associated term. PHB (polyhydroxybutyrates) an intracellular biopo...

Abstract Background and Objectives Proliferation and differentiation of mesenchymal stem cells (MSCs) in culture media requires the presence of nutrient supplements such as bovine fetal serum (FBS), which increases the risk of prion/xenotic infections. Platelet lysates as a rich source of growth factors and cytokines can replace FBS in cell culture medium. This study aimed to compare the effe...

Young African Americans have not participated as long as their white counterparts in the media culture industry (Nightingale 1993). In truth, it is difficult to discern a substantive relationship between black youth and the mass media prior to the 1960s. The initial exclusion of blacks from popular media culture is attributable to two main factors: 1) a lack of discretionary income on the part ...

After obtaining informed consent, biopsy specimens of bone were obtained from the posterior iliac crest of patients with myeloma or other neoplastic diseases with a Wester man Jenson needle. These biopsy specimens ranged from 0.3 to 0.6 cm long. The bone explants were cultured in 2 thin-film culture systems (7-9); (a) an automated unit capa ble of being programmed for proportional media exchang...

Bacillus subtHis secretes a variety of proteins directly into the culture medium at high levels.1} It is therefore possible to cause efficient secretion of useful foreign proteins using the expression and secretion systems of B. subtHis. A large number of both procaryotic and eucaryotic genes have successfully been fused t'o several secretion vectors.2~8) In most cases, however, the systems yie...