PURPOSE To review the epidemiology, risk factors, microbiologic spectrum, and treatment of microbial keratitis during a five-year period at an urban public hospital with comparison to similar findings a decade earlier at the same hospital. METHODS Retrospective chart review in the 5-year interval 2009 through 2014 compared to previously reported cases 2000 through 2004 [Eye & Contact Lens 33(...

AIMS To determine the quantitative relation between the major risk factors for microbial keratitis of previous ocular surface disease and contact lens wear and central and peripheral infiltration, often associated with ulceration, in order to establish a rational chemotherapeutic management algorithm. METHODS Data from 55 patients were collected over a 10 month period. All cases of presumed m...

The TSH-dependent expression of amyloid precursor-like proteins and the secretion of their ectodomain (sAPP) in rat thyroids coincide with increased rates of thyrocyte proliferation. To analyze whether the secretion of sAPP and the proliferation of thyrocytes are regulatorily linked, we employed [3H]thymidine or 5-bromo-2'-deoxyuridine assays and found that conditioned culture medium stimulated...

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as fi lling materials. the aim of this study was to evaluate the cytotoxic effect of a new copaiba oil-based root canal sealer (biosealer [bs]) on osteoblast-like osteo-1 cells. materials and methods: the experimental groups were formed according to the culture medium cond...

Catabolism of sulfated mucopolysaccharide by normal human fibroblasts in culture is progressively inhibited as the pH of the growth medium is raised from 6.8 to 8.0. The final cell density increases with the change in pH. The capacity to degrade mucopolysaccharide is rapidly restored by lowering the pH, and this reactivation does not require protein synthesis. Such pH dependence is not observed...

The influence of collagen gels on the orientation of the polarity of epithelial thyroid cells in culture was studied under four different conditions. (a) Isolated cells cultured on the surface of a collagen gel formed a monolayer. The apical pole was in contact with the culture medium and the basal membrane was attached to the substratum. (b) Isolated cells embedded inside the gel organized wit...

Tissue engineering is defined as the designing and engineering of structures to rebuild and repair a body damaged tissue. Scaffolding Poly Hydroxy Butyrate Valraty (PHBV) has shown good biocompatibility and biodegradable properties. Nanofibers have improved the performance of biomaterials, and could be considered effective. One of the important methods for designing nanofiber scaffold is the el...

PURPOSE To measure the extent of microbial hand contamination among ophthalmologists during routine clinic practice and examine its association with hand cleansing practices and beliefs, glove use, and patient load. METHODS This was a single-masked analysis of resident and transient flora of ophthalmologists before and after patient examination and after handwashing by agar imprints of the do...