Abstract Melon (Cucumis melo L.) is an important vegetable crop that has extensive history of cultivation. However, the genome wild and semi-wild melon types can be used for analysis agronomic traits not yet available. Here we report a chromosome-level T2T assembly 821 (C. ssp. agrestis var. acidulus), with two haplotypes ~373 Mb ~364 Mb, respectively. Comparative discovered significant number ...