Calciphylaxis, necrotizing livedo reticularis or calcific uraemic arteriolopathy (CUA) is an almost always fatal disease characterized by painful skin lesions localized on the lower extremities. This complication is typically observed in patients with end-stage renal disease on dialysis or after recent kidney transplantation [1,2]. Key histopathological features include calcification of the med...

The p53 tumor-suppressor gene encodes a nuclear phosphoprotein with cancer- inhibiting properties. The most probable cancerous mutations occur as point mutations in exons 5 up to 8 of p53, as a base pair substitution that encompasses CUA and GAT sequences. As DNA drug design represents a direct genetic treatment of cancer, in the research reported computational drug design was carried out to ex...

An amber suppressor tRNA was prepared in vitro by runoff transcription with T7 RNA polymerase. Both full-length tRNA and truncated tRNA lacking the 3' terminal pCpA from the acceptor stem could be synthesized from the same DNA template. Truncated runoff suppressor tRNA could be enzymatically ligated to phenylalanyl-pCpA to generate aminoacylated full-length suppressor tRNA (Phe-tRNA(CUA)). Phe-...

The three mitochondrial-encoded proteins, COX1, COX2, and COX3, form the core of the cytochrome c oxidase. Upon synthesis, COX2 engages with COX20 in the inner mitochondrial membrane, a scaffold protein that recruits metallochaperones for copper delivery to the CuA-Site of COX2. Here we identified the human protein, TMEM177 as a constituent of the COX20 interaction network. Loss or increase in ...

Abstract What can government do to secure environmental sustainability and resilient communities? We discuss an emerging policy approach in natural tourism resource management the Okinawa Prefecture using case of ‘Conservation Use Agreement’ (CUA, hereafter) defined Act on Special Measures for Promotion . The CUA is certification by Governor Prefecture, which endorses self-regulation concluded ...

High nutrient loads, which damage ecological assets, are a widespread problem for many Australian river catchments. These negative effects can be mitigated by land use changes and/or intervention measures such as the construction of artificial wetlands and water treatment plants. Usually budget constraints limit the number of measures that can be implemented by the catchment management authorit...

Maturation of cytochrome oxidases is a complex process requiring assembly of several subunits and adequate uptake of the metal cofactors. Two orthologous Sco proteins (Sco1 and Sco2) are essential for the correct assembly of the dicopper CuA site in the human oxidase, but their function is not fully understood. Here, we report an in vitro biochemical study that shows that Sco1 is a metallochape...