Abstract Study question Which parameters are the most predictive of embryo survival rate in oocyte recipient cryotransfer cycles? Summary answer We have identified that only day vitrification and grade expansion an impact on What is known already Embryo a well stablished technique has led to rates around ±96%. Recent studies, based vitrification, blastocyst quality, trying identify which vitrif...

Abstract Study question Can a deep learning algorithm determine the implantation likelihood of vitrified blastocysts from single time-point post-warming time-lapse images obtained EmbryoScope? Summary answer A significant positive correlation was found between score given by and rate frozen analyzed. What is known already Despite safety success vitrification technique, many best morphologically...

Background: Gamete cryopreservation is an inseparable part of assisted reproductive technology, and vitrification is an effective approach to the cryopreservation of oocytes. The aim of this study was to investigate vitrification effects on the expression levels of mitochondrial transcription factor A (Tfam) and mitochondrial-encoded cytochrome c oxidase subunit 1 (Cox1) in mouse metaphase II o...

Abstract Study question Is the human egg spindle integrity impaired by vitrification and thawing procedures? Summary answer Cryoprotective-caused dehydration stabilizes MII spindle, but re-entry of water during induces depolymerization microtubules, thus promoting instability division apparatus. What is known already Freezing sperm preimplantation embryos have become routine IVF procedures with...

STUDY QUESTION Is the attachment of biofunctionalized polysilicon barcodes to the outer surface of the zona pellucida an effective approach for the direct tagging and identification of human oocytes and embryos during assisted reproduction technologies (ARTs)? SUMMARY ANSWER The direct tagging system based on lectin-biofunctionalized polysilicon barcodes of micrometric dimensions is simple, s...

The history of IVF is synonymous with several millstones and each of these have led to a revolution in our understanding of human fertility or causes of male and female infertility, as well as introducing new treatment protocols for infertility. It can undoubtedly be claimed that one of the best of these was the introduction of ultrarapid glass-like solidification of a solution without ice-crys...

Growing evidence of successful outcomes achieved with the oocyte vitrification technique has greatly contributed to its application in field fertility preservation (FP). The population that can benefit from FP includes women at a risk losing their ovarian function because either iatrogenic causes or natural depletion reserve. Therefore, oncological patients and healthy who wish delay motherhood...