Recently detected but unidentified cytokinin activity in crown gall tumor tissue from Vinca rosea L. grown on media containing sources of reduced nitrogen has now been attributed to two adenine-type cytokinins. These compounds are glucopyranosyl derivatives of zeatin and ribosylzeatin. The substitution in each case is on the isopentenyl chain of the parent compound. Neither of these compounds h...

objective: methanol extract of bran and unpolished grain of two traditional aromatic rice genotypes viz. kalijira and chinigura were assayed for their activity on the growth and initiation of crown-gall tumors on potato disks. materials and methods: three agrobacterium tumefaciens (a. tumefaciens) strain atsl0105, atta0112, and atac0114 were used as the tumor forming agent. collected rice was s...

An amine, after dansylation, has been isolated from Nicotiana tabacum crown gall tumours for the first time and characterized as 4-hydroxy-3-methoxy-beta-phenylethylamine (3-methoxytyramine). The compound cannot be detected in differentiated N. tabacum tissues but appears in the corresponding callus controls. Its concentration is further increased 5-42-fold when N. tabacum is transformed with a...

We have examined the response of the hormone-resistant mutants axrl and axr2 of Arabidopsis thaliana to inoculation by Agrobacterium tumefaciens and Agrobacterium rhizogenes. Our results indicate that recessive mutations in the axrl gene affect the frequency of tumor formation after inoculation with either Agrobacterium strain. In addition, tumors produced on axrl plants were smaller than those...

Cultivar of roses (Rosa spp.) has been grown in greenhouses in Qazvin region of Iran for local markets. Agrobacterium tumefaciens strains were isolated and identified from six different samples of roses plants imported from the Netherland to Iran. During August and September of 2012, nearly 2-5% of rose plants in two different greenhouses in the province of Qazvin were observed with crown gall ...

Shim, J.-S., Farrand, S. K., and Kerr, A. 1987. Biological control of crown gall: Construction and testing of new biocontrol agents. Phytopathology 77:463-466. Transfer deficient (Tra-) Tn5 insertion mutants of the agrocin 84 plasmid strain K84 in controlling crown gall. Strains of Agrobacterium radiobacter pAgK84 were transformed into two chromosomal backgrounds and the which contained a high ...

Anguina pacificae is distributed along a narrow strip on the Pacific coast of Northern California where it forms galls on the shoots of Poa annua and causes significant damage to golf course greens. Methods were developed for the continuous propagation of A. pacificae on P. annua in growth chambers, and they were used to examine the life cycle and host-parasite relationships of the nematode. At...

The effect of high temperature treatments on the early stages of crown gall tumorigenesis in sunflowers was investigated. Treatments of 32 C initiated at various times during the first ten days after infection had a similar effect on tumors induced by Agrobacterium tumefaciens strains B(6) and C58. Tumor growth was sensitive to 32 C until 60 to 72 hours after infection and was stimulated by 32 ...

The respiratory processes of malignant tissues of animals and human beings have long been considered to differ in important respects from those of nonmalignant tissues of the same creatures. This difference expresses itself in several ways, especially in a low R.Q. (0.8 or less) and a decided anaerobic tendency to accumulate acid products, lactic acid in particular. It seems possible, indeed, i...

Crown gall disease, caused by the soil bacterium Agrobacterium tumefaciens, results in significant economic losses in perennial crops worldwide. A. tumefaciens is one of the few organisms with a well characterized horizontal gene transfer system, possessing a suite of oncogenes that, when integrated into the plant genome, orchestrate de novo auxin and cytokinin biosynthesis to generate tumors. ...