A centrifugal analyzer method was developed for measuring the MB isoenzyme of creatine kinase (EC 2.7.3.2) in serum by use of a specific immunochemical technique that avoids interference from CK-BB and adenylate kinase. Enzymatic activity was measured kinetically at 30 degrees C with an optimized reagent containing creatine phosphate as substrate. The precision (CV) of the assay was 5 to 12 per...

BACKGROUND Current literature suggests that myocarditis is a common event during influenza infection, occurring with a prevalence rate of up to 10%, but these studies have relied on relatively nonspecific techniques of varying sensitivities for the detection of myocyte injury. Using measurement of cardiac troponins I and T, this study sought to determine the prevalence of myocarditis in a large...

Objective: Diabetes mellitus is a metabolic disorder associated with impaired serum skeletal muscle pain enzymes levels. The purpose of this study was to investigate the effect of 8 weeks aerobic training with and without vitamin C consumption on serum levels of Creatine Kinase (CK) in type 2 diabetic patients (T2DM). Materials and Methods: In this clinical trial 45 women (30-45 years) with T2...

The purpose of the present study was to (a) examine player-movement patterns to determine total distance covered during competitive Rugby League match play using global positioning systems (GPSs) and (b) examine pre, during, and postmatch creatine kinase (CK) and endocrine responses to competitive Rugby League match play. Seventeen elite rugby league players were monitored for a single game. Pl...

INTRODUCTION The aim of this research was to create a laboratory instrument for the estimation of post-transplant lymphocele origin. It is based on the enzymatic activity of creatine kinase (CK) in the lymphocele content. MATERIAL AND METHODS A total of 120 lymph samples from different retroperitoneal regions were obtained from non-transplanted patients; equal numbers from the iliac region, r...

Myofibril-bound creatine kinase EC 2.7.3.2 (CK), a key enzyme of muscle energy metabolism, has been selected for studies of conformational changes that underlie the cellular control of enzyme activity. For fluorescence spectroscopy measurements, the CK molecule was double-labeled with IAF (5-iodoacetamidofluorescein) and ErITC (erythrosin 5'-isothiocyanate). Measurement of fluorescence resonanc...

PURPOSE Angiotensin-converting enzyme (ACE) inhibitors show beneficial long-term hemodynamic effects in chronically infarcted hearts. The purpose of this study was to test whether prevention of the deterioration of mechanical function by ACE inhibitors is related to beneficial effects on high-energy phosphate metabolism that is deranged in heart failure. METHODS Twelve-week old rats were rand...

Extracts of normal brains obtained at autopsy and cerebrospinal fluid (CSF) from patients with global brain ischemia were analyzed for creatine kinase (CK; EC 2.7.3.2) isoenzymes. We used both qualitative and quantitative assays (electrophoresis and immunoinhibition). Brain extracts contained CK-BB isoenzyme and mitochondrial CK. In 54 CSF samples free of blood contamination and with total acti...

Background: Early diagnosis and proper treatment of patient with acute coronary syndrome (ACS) and ischemic heart disease are important in determining prognosis, preventing adverse effects, and may even save lives. In this study, the level of pregnancy-associated plasma protein-A (PAPP-A) in ACS patients was compared with the control group, in addition to cardiac Troponin (cTn) and creatine kin...

BACKGROUND: Cardiovascular system is a very importantorgan that plays a vital role in tissue function. In farm animals,the growth and high milk production depends on function ofcardiovascular system. OBJECTIVES: Cardiovascular health inhigh and low-yielding dairy cows was investigated. METHODS:Fifty 4-year-old high- yielding Holstein dairy cows and fifty 4-year-old low- yielding Holstein dairy ...