Recent investigations tend heavily to support the view that ascorbic acid oxidase is a specific enzyme containing copper as the prosthetic group of a blue-green conjugated protein having a molecular weight of about 150,000 (1, 2). Little is known at the present time concerning either the nature of the copper-protein bond in the enzyme or the function of the enzyme copper in the catalytic proces...

1. The amino acid sequence of peptide (1-24) obtained from limited peptic digestion of bovine serum albumin has been determined from characterization of its tryptic and chymotryptic peptides. The 3 histidyl residues have been shown to occupy positions 3,9, and 18. 2. Hydrogen ion titration of this peptide with and without copper ions indicates that at neutral pH there are two copper(binding sit...

Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu(2+) was studied using DNA microarrays and revealed 20 genes that showed a ≥ 3-fold increased mRNA level, including cg3281-cg3289. Several genes in this genomic region code f...

Cox17 is a 69-residue cysteine-rich, copper-binding protein that has been implicated in the delivery of copper to the Cu(A) and Cu(B) centers of cytochrome c oxidase via the copper-binding proteins Sco1 and Cox11, respectively. According to isothermal titration calorimetry experiments, fully reduced Cox17 binds one Cu(I) ion with a K(a) of (6.15 +/- 5.83) x 10(6) M(-1). The solution structures ...

Copper (II) is a biologically active essential ion and it forms the active centers in several metalloenzymes and proteins. Its chelating ability and positive redox potential allow participation in biological transport reactions. The copper atom transforms between the Cu(I) and Cu(II) oxidation states in redox reactions involving copper containing enzymes. This is an essential factor for many of...

We describe an efficient, bubble-free nanoscale motor consisting of a copper-platinum (Cu-Pt) segmented rod that operates as a nanobattery in dilute aqueous Br(2) or I(2) solutions. The motion of the rod is powered by self-electrophoresis caused by redox reactions occurring on the two different metal segments. Asymmetric ratchet-shaped pure copper nanorods were also found to rotate and tumble i...

Langmuir probe, optical emission spectroscopy, and biased quartz crystal microbalance measurements were used to investigate an argon and copper plasma used for ionized physical vapor deposition of copper. Copper vapor generated by a magnetron sputter discharge is ionized upon passing through an argon discharge excited by an internal rf induction antenna. Argon plasma characteristics such as ele...

Copper is an essential metal for life, but toxic at high concentrations. In mammalian cells, two copper transporters are known, CTR1 and CTR2. order to gain insights on the possible influence of import pathway cellular responses copper, challenges were compared: one with ion, which likely use preferentially CTR1, a copper-polyacrylate complex, will be internalized via endosomal A model system c...

the objectives of this investigation are the studies on the effect of copper ion on photolytic degradation of ethylenediaminetertraaceticacid (edta), the effect of edta on electrolytic recovery of copper as well as the introduction of a novel combined photolytic and electrolytic cell system for simultaneous recovery of copper and the degradation of edta.in this experimental study, a photolytic ...