The bioleachings of chalcopyrite ore were compared after inoculating different cultures enriched from the original acid mine drainage sample. The results showed that the higher bioleaching performance was achieved for inoculation with the enrichment D (0.5 % S, 2 % iron and 1 % chalcopyrite) compared to other enrichment systems. The generated ferric precipitation during bioleaching had a key in...

a simple and sensitive method is described for the pre-concentration by cloud point extraction followed by atomic absorption spectrometric determination of copper ion in real samples. at a solution ph of 5.5, 5, 5-diphenylimidazolidine-2, 4-dione (phenytoin) (dfid) and tritonx-114 were used as hydrophobic ligand and non-ionic surfactant. the adopted concentrations for dfid, triton x-114 and hno...

The extraction of metals from supports based on calcium phosphate was investigated; we conducted experiments with metal solutions at various concentrations, and using two carriers’ calcium phosphate hydroxyapatite and hydroxyapatite in the presence of polyethylene glycol polymer. The results show a metal retention of selectivity with excellent yields. The kinetic study shows that the extraction...

The leaching behaviors of gold and silver from a complex sulfide concentrate in copper-tartrate-thiosulfate solutions were investigated this paper. Experimental parameters, including temperature, initial pulp pH, concentration copper, tartrate, thiosulfate, systematically studied. system exhibits promising performance dealing with the concentrate. Thiosulfate consumption could be greatly reduce...

Mine waters and tailings generated from mining and mineral processing activities often have detrimental impact on the local environment. One example is acid mine drainage, in which sulphides in the mining waste react with water and oxygen to produce an acidic environment that subsequently dissolves host rock minerals from the waste containing toxic metals and trace elements. Copper is one such ...

A new method for the determination of trace copper(II) in food samples by Cloud Point Extraction(CPE) combined with Flame Atomic Absorption Spectrometry(FAAS) is presented and evaluated. The method is based on the fact that hydrophobic complex of copper(II) with sodium diethyldithiocarbamate (DDTC) was formed at pH 6.0 and subsequently the hydrophobic complex was extracted into surfactant-r...

Metallothionein was purified under anaerobic conditions from livers of human fetuses ranging from 19 weeks gestational age to term. Homogeneous metallothionein obtained in the absence of reducing agents from the soluble fraction of the tissue which contained 24% and 85% of the total liver copper and zinc, respectively, had less than 1 g atom of copper and about 3.1 g atoms of zinc/mol. Extracti...

Fe is an impurity in most leach liquors. Its coexistence with copper in leaching solution of chalcopyrite (CuFeS2) which is the most important mineral of copper creates major extraction problems. Hydrochloric acid dissolves both copper and iron during chloride leaching of this mineral. Separation of Fe from Cu is thus necessary to obtain pure copper. This paper presents a novel metho...

A system for separation of zinc traces from large amounts of cadmium is proposed in this paper. It is based on the solid-phase extraction of the zinc in the form of thiocyanate complexes by the polyurethane foam. The following parameters were studied: effect of pH and of the thiocyanate concentration on the zinc extraction, shaking time required for quantitative extraction, amount of PU foam ne...

BACKGROUND The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reporte...