We present the draft genome sequence of Legionella massiliensis strain LegA(T), recovered from a cooling tower water sample, using an amoebal coculture procedure. The strain described here is composed of 4,387,007 bp, with a G+C content of 41.19%, and its genome has 3,767 protein-coding genes and 60 predicted RNA genes.

Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps related to infectivity, in Legionella populations.

We report here the complete genome sequences of two of the earliest known strains of Legionella pneumophila subsp. fraseri Detroit-1 is serogroup 1 and was isolated from a lung biopsy specimen in 1977. Dallas 1E is serogroup 5 and was isolated in 1978 from a cooling tower.

A negative enrichment procedure was developed which was capable of isolating Legionella pneumophila directly from seeded air-conditioning cooling tower water onto laboratory media. This procedure was based on an 8-h incubation under conditions that were bactericidal to the indigenous water microflora but merely bacteriostatic to L. pneumophila.

Abstract A theoretical study is developed to examine the feasibility of using cooling tower and coil with direct evaporative cooler in highest summer temperature Karbala/Iraq. This due inability reducing outside air efficiency reduction mechanical vapor compression systems despite its high electrical energy consumption at temperatures. The water cooled a higher than outdoor wet-bulb by (3.9 $$^...

Nuclear and other large-scale energy-producing plants must include systems that guarantee the safe discharge of residual heat from the industrial process into the atmosphere. This function is usually performed by one or several cooling towers. The amount of heat released by a cooling tower into the external environment can be quantified by using a numerical simulation model of the physical proc...

Water-cooled chiller systems have typically been designed around entering condenser water temperatures of 85°F with a Optimization of Water Cooled Chiller – Cooling Tower Combinations The warm water leaving the chilled water coils is pumped to the evaporator of the chiller, where the unwanted heat from the building is transferred by the latent heat of vaporization of the refrigerant. The compre...

Our survey of cooling tower water demonstrated that the highest density of legionellae, >/=10 CFU/100 ml, appeared in water containing protozoa, >/=10 MPN/100 ml, and heterotrophic bacteria, >/=10 CFU/100 ml, at water temperatures between 25 and 35 degrees C. Viable counts of legionellae were detected even in the winter samples, and propagation, up to 10 CFU/100 ml, occurs in summer. The counts...