Noroviruses (NoVs) are the most commonly identified cause of outbreaks and sporadic cases of acute gastroenteritis. We evaluated and optimized NoV-specific TaqMan real-time reverse transcription (RT)-PCR assays for the rapid detection and typing of NoV strains belonging to genogroups GI and GII and adapted them to the LightCycler platform. We expanded the detection ability of the assays by deve...

| The paper deals with the formal speciication of a general-purpose multimedia synchronization mechanism, known as the conditional delivery mechanism capable of addressing both intra-and inter-stream synchronization issues. The proposed mechanism, initially introduced in 3, 11], is based on the identiication of causal relations among information units of one or several streams and has the purpo...

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 gen...

This paper presents a novel landmark generating method for robust multi-robot localization and tracking. Although the conventional approaches are able to generate a number of landmarks, they often tend to misjudge or ignore some landmark in particular environment. Analyzing those methods, we suggest the new landmark that is referred to here as Rotating Triangles (RT) landmark. RT landmark imple...

In this paper, several energy recovery circuits (ERC) for the plasma display panel (PDP) are reviewed and classified in a manner of operation characteristics. A typical ERC using the Regenerative Transformer (RT) is introduced and compared with conventional ones. RT-ERC can achieve zero-voltage-switching (ZVS) for main full-bridge switches and zero-current-switching (ZCS) for the resonant switc...

OBJECTIVE A novel rapid reverse transcriptase (RT) recombinant HIV-1 drug-susceptibility assay was developed to evaluate resistance to RT inhibitors. MATERIAL AND METHODS HIV-1 RTs from five treatment-naive and 10 highly active antiretroviral therapy-experienced patients were evaluated. HIV-1 isolates recovered by culturing peripheral blood mononuclear cells from patients were used in the con...

Recently developed PCR-based reverse transcriptase (RT) assays are useful in the detection of retroviruses since they are approximately a millionfold more sensitive than conventional RT assays. However, these assays are both labor- and time-intensive. The previously described product-enhanced reverse transcriptase (PERT) assay involves a two-step RT-PCR followed by detection and quantitation of...

Respiratory syncytial virus (RSV) is one of the most important virus respiratory pathogens in infants and young children. A rapid and sensitive diagnosis is essential to focus any outbreak due to this virus. A real-time RT-PCR method was designed using a primer/probe pair from the F gene. Simultaneously with nested RT-PCR and antigen ELISA, 71 consecutive specimens from hospitalized children wi...

A promising cost-effective approach to realizing RT ADS's is to use the emerging technology for RT distributed component/object structuring. One such technology being explored by the author and his research collaborators is the the Time-Triggered MessageTriggered Object (TMO) structuring scheme [Kim94, Kim97b]. The TMO scheme is intended to facilitate RT distributed software engineering in a fo...

In situ RT-PCR detects and amplifies mRNA (cDNA) while obtaining spatial information of gene expression. When the intended use is an ultrastructural analysis of morphology, the procedure may be technically challenging and quality of tissue dramatically altered by proteolytic digestion and extreme astringency and temperature conditions. We describe a low-damaging protocol of in situ RT-PCR combi...