We identified 1219 articles published in 2006 that described work performed using commercial optical biosensor platforms. It is interesting to witness how the biosensor market is maturing with an increased number of instrument manufacturers offering a wider variety of platforms. However, it is clear from a review of the results presented that the advances in technology are outpacing the skill l...

An amperometric benzoic acid-sensing inhibitor biosensor was prepared by immobilizing mushroom (Agaricus bisporus) tissue homogenate on a Clark-type oxygen electrode. The effects of the quantity of mushroom tissue homogenate, the quantity of gelatin and the effect of the crosslinking agent glutaraldehyde percent on the biosensor were studied. The optimum concentration of phenol used as substrat...

A novel biosensor based on carbon nanofibers/diamine oxidase was prepared by modification of screen-printing electrodes. The enzyme diamine oxidase was immobilized onto the carbon nanofibers modified carbon screen-printed electrodes by drop and dry method followed by cross-linking reaction with glutaraldehyde. The presence of norepinephrine in analyzed samples was correlated to increasing of ca...

• Two competing and opposing processes control the sensing response of NiO to ethanol. surface orientation strongly determine its interaction with ethanol at RT. Charge transfer adsorbed molecules dominates in anomalous response. Ethanol decomposition is responsible for normal The shows faster dynamics better sensitivity Conductometric gas sensors based on metal oxide semiconductors (MOS) usual...

We present here the phasor approach to biosensor Förster resonance energy transfer (FRET) detection by fluorescence lifetime imaging microscopy (FLIM) and show that this method of data representation is robust towards biosensor design as well as the fluorescence artifacts inherent to the cellular environment. We demonstrate this property on a series of dual and single chain biosensors, which re...

We present a quantitative overhead analysis for effective task migration in biosensor networks. A biosensor network is the key technology which can automatically provide accurate and specific parameters of a human in real time. Biosensor nodes are typically very small devices, so the use of computing resources is restricted. Due to the limitation of nodes, the biosensor network is vulnerable to...

A simple visual ethanol biosensor based on alcohol oxidase (AOX) immobilised onto polyaniline (PANI) film for halal verification of fermented beverage samples is described. This biosensor responds to ethanol via a colour change from green to blue, due to the enzymatic reaction of ethanol that produces acetaldehyde and hydrogen peroxide, when the latter oxidizes the PANI film. The procedure to o...

The optimization-based quantitative determination of multianalyte concentrations from biased biosensor responses is investigated under internal and external diffusion-limited conditions. A computational model of a biocatalytic amperometric biosensor utilizing a mono-enzyme-catalyzed (nonspecific) competitive conversion of two substrates was used to generate pseudo-experimental responses to mixt...

Pseudomonas fluorescens cultures produce fluorescent siderophores. By utilizing optimal conditions for maximizing siderophore production in shake flask cultures of P. fluorescens, we report successful characterization of the culture broth supernatant as a robust ferric ions biosensor. For characterizing the ferric ions biosensor, we tested the effects of pH, buffers, different ferric salts and ...

Microelectrode amperometric biosensors are widely used to measure concentrations of analytes in solution and tissue including acetylcholine, adenosine, glucose, and glutamate. A great deal of experimental and modeling effort has been directed at quantifying the response of the biosensors themselves; however, the influence that the macroscopic tissue environment has on biosensor response has not...