lipofectamine ltx is a cationic based transfection reagent that offers high levels of different transgenes expression in a huge number of cell types in vitro utilizing an easy and rapid protocol. the level of transgenes expression depend on some experimental variables containing cell density, lipofectamine and dna concentrations, lipofectamine -dna complexing time, and the presence or absence o...

The somatic cell hybrid method has been used to study the number and different types of human genes involved in the expression of adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) in normal cells and cells from a patient with ADA-deficient severe combined immunodeficiency disease (SCID). Genetic and biochemical characterization of ADA in SCID and the ADA tissue-specific isozymes i...

Fluorescence quenching of the fluorescence is a useful method to determine copper complexing properties of the dissolved organic matter. This technique provides express results often by choosing one or two excitation-emission fluorescence measurement for a straight range of concentration. That why it is not representative of the whole sample. On another hand, using total luminescence spectra gi...

Veterinary diagnostic test kits are used in clinical practice and production medicine as a basis for prescribing vaccination and/or treatment regimens. These kits are also used by state and federal animal health officials to control or regulate the movement of animals. The regulation of such kits, which generally takes the form of establishing requirements for licensure, ensures that the kits c...

The objective of this study was to determine the complexing capacity of four types of powdered commercial milks with copper(II) using square wave adsorptive stripping voltammetry. Two were types of cow milk adapted for babies under one year (A and B), one was soymilk (C) and the other was normal milk (D). Milk solutions were prepared following the instructions shown on the milk container, and t...

Marine microalgae (Emiliania huxleyi) were grown in seawater enriched only with nitrogen and phosphorus, without control of free metal concentrations using synthetic chelators. Complexing ligands and thiol compounds were determined by cathodic stripping volt-ammetry. Copper was added to these cultures, and ligands were produced in response to the copper addition. Parallel measurements of thiols...