Infectious bronchitis (IB) is a very contagious disease caused by a coronavirus (IBV). In chickens, thevirus affects the respiratory, reproductive, and urinary systems. This study was carried out to determine theseroprevalence of anti-IBV antibodies in domestic village chickens. Serum samples of 300 domestic villagechickens from Esfahan (centeral Iran) were collected and examined for the presen...

BACKGROUND The demonstration of proteinase 3 specific antineutrophil cytoplasmic antibodies (PR3-ANCA), and the estimation of antibody values are useful in the diagnosis and management of patients with Wegener's granulomatosis (WG). However, external quality assessment programmes suggest that PR3-ANCA binding varies in different assays. AIM To demonstrate variations in PR3-ANCA binding in dif...

Celiac disease (CD) is an enteropathy mediated by immunological mechanisms triggered by the interaction of gluten with the intestinal mucosa in affected individuals [1]. The consequences of this condition are the intestinal extensions leading to malabsorption and malnutrition [2]. The only currently effective health strategy for affected consumers is to avoid glutencontaining products, and such...

Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...

infectious bronchitis (ib) is a very contagious disease caused by a coronavirus (ibv). in chickens, thevirus affects the respiratory, reproductive, and urinary systems. this study was carried out to determine theseroprevalence of anti-ibv antibodies in domestic village chickens. serum samples of 300 domestic villagechickens from esfahan (centeral iran) were collected and examined for the presen...

Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. With this method the cloned target gene can be altered by substitution, deletion or insertion of a few bases directly into a plasmid. It works by simply amplifying the whole plasmid, in a non PCR-based thermocycling reaction. During the reaction mutagenic primers, carrying...