Secreted signaling molecules such as Wingless (Wg) and Decapentaplegic (Dpp) organize positional information along the proximodistal (PD) axis of the Drosophila wing imaginal disc. Responding cells activate different downstream targets depending on the combination and level of these signals and other factors present at the time of signal transduction. Two such factors, teashirt (tsh) and homoth...

Background. Anterior disc displacement without reduction of the temporomandibular joint (TMJ) is a relatively common finding, and generally, the treatment option for these patients involves surgery, which can include multiple procedures such as arthrocentesis, eminectomy, vertical subsigmoid osteotomy, or meniscopexy. Objective. The purpose of this study is to clinically evaluate the effectiven...

Neurogenesis in Drosophila eye imaginal disc is controlled by interactions of positive and negative regulatory genes. The basic helix-loop-helix (bHLH) transcription factor Atonal (Ato) plays an essential proneural function in the morphogenetic furrow to induce the formation of R8 founder neurons. Bar homeodomain proteins are required for transcriptional repression of ato in the basal undiffere...

Ovaries of adult Japanese quails were exposed in vivo to the acid bisazo dye trypan blue (TB) which binds to plasma albumin, the plasma precursor of the yolk protein alpha-livetin. By a combination of fluorescence microscopy and electron microscopy alpha-livetin could be localized in the subdroplets of intermediary and yellow yolk spheres. Trypan blue alters vitellogenesis in the non-disc regio...

BACKGROUND Recent evidence suggests that co-clustering of Fas/CD95 death receptor and lipid rafts plays a major role in death receptor-mediated apoptosis. METHODOLOGY/PRINCIPAL FINDINGS By a combination of genetic, biochemical, and ultrastructural approaches, we provide here compelling evidence for the involvement of lipid raft aggregates containing recruited Fas/CD95 death receptor, Fas-asso...

background & aim: low back pain is broadly documented as one of the most widespread pathologies in the advanced domain. although the reasons of low back pain are uncountable, it has been meaningfully related to intervertebral disc degeneration. present therapies for intervertebral disc (ivd) degeneration such as physical therapy and spinal fusion reduce symptoms' severity, but do not treat the ...

introduction: due to limitations of current treatments for degenerative disc disease, arthroplastic methods to repair the diseased disc have been proposed. the artificial disc is a mobile implant for degenerative disc replacement that attempts to lessen the degeneration of the adjacent elements following interbody fusion procedures. because the success of artificial disc replacement depends on ...

The reverse genetics technology for bluetongue virus (BTV) has been used in combination with complementing cell lines to recover defective BTV-1 mutants. To generate a potential disabled infectious single cycle (DISC) vaccine strain, we used a reverse genetics system to rescue defective virus strains with large deletions in an essential BTV gene that encodes the VP6 protein (segment S9) of the ...

Previous studies indicate that lumbar radicular pain following disc herniation may be associated with release of several pro-inflammatory mediators, including interleukin-1 (IL1). In the present study, we examined how genetic variability in IL1A (rs1800587 C>T), IL1B (rs1143627 T>C) and IL1RN (rs2234677 G>A) influenced the clinical outcome the first year after disc herniation. Patients (n = 258...

Lab-on-a-Disc (LoaD) has great potential for applications in decentralised bioanalytical testing where speed and robustness are critical. Here, a disc-shaped microfluidic chip is rotated to pump liquid radially outwards; thus, all structures must be fitted into the available radial length. To overcome this limitation, several centripetal pumping technologies have been developed. In work, we com...