To demonstrate that two structurally similar chemicals can be extracted from a complex matrix and then separated from each other on the basis of their relative affinities for an antibody, an automated column-switching system was used, incorporating on-line, high-performance immunoaffinity chromatography (HPIAC). A high-affinity monoclonal antibody (Mab Sara-95) against the fluoroquinolone saraf...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

calystegines were isolated from the root cultures of physalis divaricata, p. pubescens, p. philadelphia,and p. philadelphia (solanaceae). calystegines of p. divaricata were identified as calystegine a3, a5, b1 and b2, with concentrations of 6.99, 4.41, 8.52, and 14.7 ?g/g in fresh mass, respectively. root cultures of p.pubescens contain calystegine a3, b1 and b2, p. philadelphia only contains c...