نتایج جستجو برای: colony assay

تعداد نتایج: 273197  

A Allahverdi, F skandari, H Nasiri, H Zare-Zardini, M Azad, M Soleimani, N Kalantari,

Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...

Feng-Yin Sun, Ming Ming, Xin-hai Cui, Yong-Toa Xu Zhoa-Long Ma

Wilms tumor (WT) is the most common pediatric malignant primary renal tumor. Carboplatin (CRB), a platinum compound is widely used in the treatment of multiple cancers including ovarian, lung, head and neck, and wilm’s tumor. However lower uptake of CRB in cancer cells and toxicity concerns in healthy cells often limited its clinical outcome. The aim of this study was to investigate the antitum...

Journal: :Cytometry. Part A : the journal of the International Society for Analytical Cytology 2010
Matthew L Clarke Robert L Burton A Nayo Hill Maritoni Litorja Moon H Nahm Jeeseong Hwang

Research involving bacterial pathogens often requires enumeration of bacteria colonies. Here, we present a low-cost, high-throughput colony counting system consisting of colony counting software and a consumer-grade digital camera or document scanner. We demonstrate that this software, called "NICE" (NIST's Integrated Colony Enumerator), can count bacterial colonies as part of a high-throughput...

Journal: :Cancer research 1985
J P de Jong P G Nikkels K G Brockbank R E Ploemacher J S Voerman

We investigated the in vitro effects of ASTA-Z-7595, ASTA-Z-7557, ASTA-Z-7654, and 4-hydroperoxycyclophosphamide (4HC) on murine stromal fibroblastoid colony-forming units, committed hemopoietic progenitors (erythroid burst-forming units and granulocyte/macrophage colony-forming units), and pluripotent hemopoietic stem cells assayed by the spleen colony-forming unit (CFU-s) assay. In general, t...

2012
Takuya Kuroda Satoshi Yasuda Shinji Kusakawa Naoya Hirata Yasunari Kanda Kazuhiro Suzuki Masayo Takahashi Shin-Ichi Nishikawa Shin Kawamata Yoji Sato

Human induced pluripotent stem cells (hiPSCs) possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical problems associated with human embryonic stem cells (hESCs). These characteristics make hiPSCs a promising choice for future regenerative medicine research. There are significant obstacles, however, preventing the clinical use of h...

Journal: :Journal of invertebrate pathology 2001
J D Evans

Nucleotide sequence analyses were used to identify acute bee paralysis virus (ABPV) and Kashmir bee virus (KBV) isolated from a single honey bee colony. Most of the bees in this colony carried KBV. Some individual bees also carried ABPV, a coexistence not yet seen between these two viruses. Implications of coinfection on viral efficacy are discussed, along with a restriction enzyme assay that c...

Journal: :Blood 1989
C A Dahl C Lindqvist

Normal mouse serum (NMS) devoid of colony-stimulating factor (CSF) was found to enhance the interleukin 3 (IL-3)-driven colony formation of bone marrow in vitro. Inclusion of NMS in bone marrow colony-forming assays resulted in greatly increased numbers of colonies and clusters following seven days incubation; however, incubation of bone marrow with NMS before the colony-forming assay had no ef...

Journal: :Tropical Journal of Pharmaceutical Research 2023

Purpose: To determine the potential effect of Chikusetsu saponin IVA (CS-IVA) on benign prostatic hyperplasia (BPH), as well mechanism action.
 Methods: Benign epithelial cell response to treatment by CS-IVA was evaluated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation assay, and cycle analysis. Flow cytometry immunoblot assay were used assess apoptosis...

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