Bovine articular cartilage explants were maintained in culture for 4 days in the presence of 25 units o f IL-1 with 0 (m), 50 ( m ) and 200 ( m ) pg/ml naproxen, and the media were analysed to determine proteoglycan ( a ) and collagenase activity (h). cultures, and this was maintained for subsequent days. Gelatinase activity showed no significant change with 1L-l at any time. TIMP activity show...

A selective, sensitive, and convenient assay for human collagenase is required because of its implication in diseases such as rheumatoid arthritis, osteoarthritis, and tumors. Here, a novel assay for human collagenase activity is described in which enzymatic degradation of native collagen or acetyl peptide is determined by using a fluorogenic reaction for oligopeptides. The oligopeptides are qu...

Rosaindica and Psidiumguajava methanol extracts were tested for their effect on urease and collagenase enzyme by using phenol hypochlorite method and gelatin digestion assay, respectively. Among the two plant extracts evaluated, the highest inhibitory activity against Jack bean urease was exerted by R. indica(47.11%), while for collagenase P. guajava showed complete inhibition of enzyme at conc...

OBJECTIVE To demonstrate the binding and inactivation action of a superabsorbent dressing on proteolytic enzymes MMP-2, MMP-9 and collagenase using an established methodology. METHOD An in vitro assay of MMP binding and collagenase inactivation has been conducted using the superabsorbent wound dressing (Eclypse; Advancis Medical UK). Dressings in this category, and other absorbents, have been...

the aim of this study was to evaluate the effect of chlorhexidine (chx) application on immediate and two-month bond strength of a filled adhesive system. methods: forty eight non-carious human third molars without enamel of occlusal surface were randomly divided into two main groups according to chx application: control and chx. each main group was further subdivided into 2 subgroups depending ...