OBJECTIVES To compare the properties of calcium phosphate precipitation on four different bioactive surface preparations and one control surface in the simulated body fluid model with added collagen type I. MATERIAL AND METHODS Blasted titanium discs were treated with four different surface modifications, alkali and heat, sodium fluoride, anodic oxidation and hydroxyapatite coating. The discs...

Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined met...

Introduction The use of scaffolds in tissue engineering is becoming increasingly important as solutions need to be found preserve human tissues such bone or cartilage. Various factors, including cells, biomaterials, cell and culture conditions, play a crucial role engineering. vivo environment the cells exerts complex stimuli on thereby directly influencing behavior, proliferation differentiati...

In order to evaluate the biocompatibility and bioactivity of recently described nanocomposite coatings, consisting of polyelectrolyte multilayers interspersed with calcium apatite crystals, extensive cell culture tests have been applied to coated and uncoated chemically etched titanium plates. The coatings had top calcium phosphate layers (PLL/PGA)10CaP[(PLL/PGA)5CaP]4 (A) or top polyelectrolyt...

collagens are the most abundant proteins in the human body. their main function is to provide structural and mechanical support for the tissues, but they are also involved in a number of other biological functions including cell attachment, migration and differentiation. collagens and gelatins are widely used in pharmaceutical and medical applications. every year, more than 50,000 tons of colla...

Acid-soluble collagen could be isolated from fish skin using acetic acid. In recent years, much attention has been paid to collagen from marine sources, mainly arising from the fact that there is no risk of contagious diseases. Moreover, by processing the fish, significant amounts of waste materials are produced which can be considered as a substitute for these collagen sources. Thunnus tonggol...

Sulfated glycosaminoglycans (GAG) are multifunctional components of the extracellular matrix and are involved in the regulation of adhesion, proliferation and differentiation of cells. The effects of GAG are mediated in general by their interactions with cations and water, and in particular by their binding to growth factors. The aim of this study was to generate artificial extracellular matric...

The extracellular matrix (ECM), constituting the follicular basal lamina and present also between follicular cells and in the follicular fluid, is believed to regulate granulosa cell (GC) function during follicular development. Ovine GCs isolated from small (1-3 mm in diameter) or large (4-7 mm in diameter) antral follicles were cultured on various pure ECM components (type I collagen, fibronec...