Dynamin, the GTPase required for clathrin-mediated endocytosis, is recruited to clathrin-coated pits in two sequential phases. The first is associated with coated pit maturation; the second, with fission of the membrane neck of a coated pit. Using gene-edited cells that express dynamin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single-molecule EGFP sensitivity and high temporal r...

Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H20 and 2H20/ Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major...

experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. ejaculates were collected from four rams. in each session, second ejaculates (n=4) were collected in a tube containing 1 ml coating buffers which were prepared by 10, 15 or 20% egg yolk plus tris-fructose. samples were pooled, centrifuged and the supernatant removed in the labora...

Object Based Image Analysis (OBIA) is an established method for analyzing multiscale and multidimensional imagery in a range of disciplines. In the present study this method was used for the 3D reconstruction of halite hopper crystals in a mudrock sample, based on Computed Tomography data. To quantitatively assess the reliability of OBIA results, they were benchmarked against a corresponding “g...

an action resulting from an object like human's damaging action can cause liability for those who possess them. the french civil law at its approval time (1804) had not predicted this principle and just in articles 1385, 1386, the liability of damages resulting from two types of objects, i.e. animals and buildings due to imposing great damages by them had been predicted by french law. howe...

Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H20 and 2H20/ Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major...

Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H20 and 2H20/ Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major...

Many studies have shown that our visual system may construct a “statistical summary representation” over groups of visual objects. Although there is a general understanding that human observers can accurately represent sets of a variety of features, many questions on how the statistical summary is computed still remain unanswered. This study investigated sampling properties of visual informatio...

Supercavitation technology has important application value in military and national defence fields because of its huge potential drag reduction, while the cavitation around underwater moving objects may be affected by surface properties objects. In this paper, supercavitation characteristics hydrodynamics a projectile with hydrophobic hydrophilic coatings were experimentally studied using high-...