the potential risk of metal accumulation in periurban agricultural areas is a matter for concern.the climate characteristics and carbonate content of calcareous agricultural mediterranean soils typical inthese areas favour metal accumulation at the surface level; however there is also a risk of potential metalmobility. our study focuses on the soil properties affecting metal mobility in these s...

We investigated, by a combined in vivo and in vitro approach, the temporal changes of islet nitric oxide synthase (NOS)-derived nitric oxide (NO) and heme oxygenase (HO)-derived carbon monoxide (CO) production in relation to insulin and glucagon secretion during acute endotoxemia induced by lipopolysaccharide (LPS) in mice. Basal plasma glucagon, islet cAMP and cGMP content after in vitro incub...

Current extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting DNA and RNA from sputum. We established a nucleic acid co-extraction method from sputum based on magnetic beads and optimized the method by evaluating influencing factors, such as the guanidinium thiocyanate (GTC) and dithiothreitol (DTT) concentrations, magnetic bead amount, incubation...

Soil respiration (Rs) is a major source of carbon emission in terrestrial ecosystems. Despite the fact that the influence of land use practice on Rs has been widely studied, the patterns and drivers on Rs of Citrus reticulata cultivation, a worldwide land use practice are unclear. In this current study, we investigated the influence of long-term cultivation of Citrus reticulata (CO) and of CO i...

Hypertrophic scars are a common complication of burn injury, yet there no medications to prevent their formation. Myofibroblasts, key cell involved in scar formation, resistant apoptosis. We have previously shown that hydroxypyridone anti-fungals can inhibit myofibroblast transformation scar-derived fibroblasts. This study aimed investigate if these drugs target established myofibroblasts. Prim...

The following observations indicate that cultured human WI-38 fibroblasts synthesize and secrete alpha2-macroglobulin into serum-free medium: (a) after incubation of cultures with [35S]L-methionine, a labeled protein appeared in the medium which was precipitated by antiserum directed against alpha2-macroglobulin; (b) after incubation of cultures with [35S]L-methionine, a major band of radioacti...

Although the utility of analyzing behavioral experience effects on neural cytochrome oxidase (CO) activity is well recognized, the behavioral correlates of endogenous differences in CO activity have rarely been explored. In male leopard geckos (Eublepharis macularius), the incubation temperature experienced during embryogenesis (IncT) and age affect CO activity in the preoptic area (POA), an ar...

A method for co-immobilizing lipase from porcine pancreas, glycerol kinase (GK) from Cellulomonas spp., glycerol-3-phosphate oxidase (GPO) from Aerococcus viridans and peroxidase from horseradish onto zirconia-coated alkylamine glass beads through glutaraldehyde coupling has been described. The co-immobilized enzymes retained 71.4% of initial specific activity with a conjugation yield of 43.6 m...

Uptake of (14)CO(2) in soils due to algae or sulfur-oxidizing bacteria was examined by incubation of soil samples with gaseous (14)CO(2) and subsequent chemical oxidation of biologically fixed radioactive isotope to (14)CO(2) for detection with a liquid scintillation counting system. The (14)CO(2) was added to the soil in the gas phase so that no alteration of the moisture or ionic strength of ...

A simple and rapid method for identifying eosinophils in tissue sections is described. The assay is based on the incubation of frozen tissue sections with phenol red and the detection of cellular fluorescence. A one-to-one relationship was observed between cells exhibiting fluorescence following exposure to phenol red and eosinophils as identified by histochemical detection of eosinophil peroxi...