Large deviations for additive path functionals of stochastic processes have attracted significant research interest, in particular the context particle systems and statistical physics. Efficient numerical `cloning' algorithms been developed to estimate scaled cumulant generating function, based on importance sampling via cloning rare event trajectories. So far, attempts study convergence proper...

conclusions this is the first report of expression of cmv cp gene in iran, which is important for the preparation of anti-cmv antibody and paving the way for the use of the virus coat protein as a nanomaterial. results the identity of the expressed protein was confirmed by immunoassays such as western blot, dot-immunobinding assay (diba) and enzyme-linked immunosorbent assay (elisa) by the use ...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

cucumber mosaic virus (cmv) belongs to the genus cucumovirus in the family bromoviridae. althaea officinalis, commonly known as hollyhocks in the family malvaceae, is an annual plant whose fruit, flower and root are used as medicinal products. in this study, five samples of hollyhocks leaves with viral symptoms were collected and cmv was detected using das-elisa from the samples. ta-alth isolat...

dna amplification using taq dna polymerase is one of the most widely used techniques in molecular biology and biotechnology. the aim of this study was to amplify the gene of this enzyme from a thermophilic bacteria called thermus aqauticus and clone it into a vector for future use. using specific primers the cdna of taq dna polymerase was amplified and ligated into the cloning vector ptz57r usi...

Human cloning and in vitro fertilisation may seem very different procedures; certainly, they are regarded very differently by many people at the present time. IVF is seen as a mainstream procedure raising few ethical problems, while cloning, or ‘reproductive’ cloning, is widely regarded as beyond the moral pale. In this paper, I argue that the two procedures have more in common than might at fi...