نتایج جستجو برای: cloned bovine embryos
تعداد نتایج: 144583 فیلتر نتایج به سال:
Mesenchymal stem cells (MSCs) reside in the bone marrow and have the potential for multilineage differentiation, into bone, cartilage, and fat, for example. In this study, bovine and porcine MSCs were isolated, cultured to determine their replication ability, and differentiated with osteogenic medium and 5-azacytine. Both bovine and porcine undifferentiated MSCs were electroporated and virally ...
background: over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (nt) into oocytes. although it has been 15 years since the generation of the first cloned mammals from somatic cells by nt, the success rate for producing live offspring by this technique is low regardless of the cell type and...
Fluorescence expression by bovine embryos was examined after pronuclear microinjection with an enhanced green fluorescent protein (EGFP) cDNA under control of the chicken beta-actin promoter and cytomegalovirus enhancer, as a first step in evaluating the applicability of EGFP for non-invasive selection of transgenic bovine embryos. After injection, developmental competence of the embryos was re...
background: the values of embryonic stem cell and cloning are evident. production of clone from embryonic stem cells can be achieved by introduction of stem cell into a tetraploid blastocyst. tetraploid blastocyst can be produced in vitro by electrofusion of 2-cell embryos. objective: the aim of this study was to assess the effect of different voltages and durations on fusion rate of bovine 2-c...
Cancer cells escape from growth control by accumulating genetic and epigenetic alterations. In rare instances, epigenetic changes alone are oncogenic. Furthermore, agents that modify DNA methylation or chromatin structure can restore a normal phenotype to cells harboring oncogenic mutations. However, it is unclear to what extent epigenetic reprogramming can reverse oncogenesis. Using somatic nu...
Several key developmental events are associated with early embryonic pregnancy losses in beef and dairy cows. These problems observed at a greater frequency pregnancies generated from in-vitro-produced bovine embryos. This review describes critical that arise during oocyte maturation, fertilization, development, compaction blastulation, cell lineage specification, elongation, gastrulation, plac...
Imprinting disorder during somatic cell nuclear transfer usually leads to the abnormality of cloned animals and low cloning efficiency. However, little is known about the role of donor cell imprinting in the development of cloned embryos. Here, we demonstrated that the imprinting (H19/Igf2) in porcine fetus fibroblasts derived from the morphologically abnormal cloned fetuses (the abnormal impri...
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