This study aimed to propagate lipote cuttings through clonal propagation using different growth hormones. The clones were raised in a non-misted closed chamber and transplanted into polyethylene bags for observation of their early growth. performance the was analyzed terms height, diameter, leaf number, chlorophyll content, area, shoot biomass, root total biomass. After three months, terminated...

Introduction Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 Eimeria spp. Methods Here, we cloned prokaryotically expressed partial sequence tenella (EtVPS29) with RT-PCR engineered strain Escherichia coli respectively. The localization i...

Background: Human epidermal growth factor receptor 2 (HER2) is over-expressed in breast, ovarian, gastric, and prostate cancers used as a tumor marker the diagnosis of cancer. Monoclonal antibodies have been diagnostic therapeutic tool against HER2. Because difficulties associated with stability complexity construct high cost antibody production, we aimed to investigate, cloning, expression HER...

Imprinting disorders induced by somatic cell nuclear transfer (SCNT) usually lead to the abnormalities of cloned animals and low cloning efficiency. Histone deacetylase inhibitors have been shown to improve gene expression, genomic methylation reprogramming and the development of cloned embryos, however, the imprinting statuses in these treated embryos and during their subsequent development re...

Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...

Antigen recognition by cytotoxic lymphocytes (CTL) 1 and by helper T cells is restricted by gene products of the major histocompatibility complex (MHC), called H-2 in the mouse (1). CTL recognize antigens in the context of Kand D-regionencoded structures, whereas helper T cells recognize antigen in the context of I-regionencoded structures. This H-2 restriction appears to be very tight: C T L s...

DNA methylation patterns are dynamic in cleavage-stage embryos of a number of mammalian species. A failure to properly recapitulate preimplantation DNA methylation patterns in embryos derived by nuclear transfer may contribute to the low efficiency of nuclear transfer in producing live offspring.