Fluorescence microscopy is commonly used to investigate disease progression in biological tissues. Biological tissues, however, are strongly scattering in the visible wavelengths, limiting the application of fluorescence microscopy to superficial (<200µm) regions. Optical clearing, which involves incubation of the tissue in a chemical bath, reduces the optical scattering in tissue, resulting in...

Molecular interactions of optical clearing agents were investigated using a combination of molecular dynamics (MD) simulations and optical spectroscopy. For a series of sugar alcohols with low to high optical clearing potential, Raman spectroscopy and integrating sphere measurements were used to quantitatively characterize tissue water loss and reduction in light scattering following agent expo...

Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has to compromise between clearing quality and fluorescence preservation. This can be a severe drawback when working with specimens exhibiting low...

Contact: [email protected] Fax: +81-78-306-3090 RIKEN Center for Developmental Biology Improved tissue clearing solution for super-resolution imaging April 7, 2016–As biologists seek to gain further insights on subcellular structures deeply embedded in various tissues, high resolution fluorescence microscopy using various fluorescent markers has emerged as a powerful tool to ‘see’ inside...

Phototherapy using sunburn spectrum ultraviolet radiation (UVB) is now a frequently utilized treatment for psoriasis that is extensive or has not responded to topical preparations. Four university centers performed a prospective randomized clinical trial to compare remission times of patients with psoriasis who continued UVB phototherapy after initial clearing with this therapy and patients who...

Sample preparation for confocal microscopy typically involves fixing and staining, followed by clearing and mounting. Clearing involves treatment with solvent to dissolve lipids and other structures that cause light scattering and reduce the ability to image through thick specimens. Mounting involves transfer of the specimen into a medium which matches the refractive index the objective is desi...

Abstract General faults in regular electrical railways system could be detect by implementing the specific protection functions such as ground fault or sensitive earth fault. Otherwise, in ungrounded electrical railway systems which using both feeding and return contact rail at the same time, fault detection cannot done with above-mentioned function. Due to recent growth in electrical railway ...

For several years, European financial markets have been the place of important mutations. These mutations have hit both stock markets themselves as well as the infrastructures including all necessary services for the transactions on financial securities. Among the market services to which the investors appeal, is the clearing of the orders, the service which allows reducing exchanged flows whil...

risk assessment of soil erosion, one of the most important land degradation problems worldwide, is very essential for land and water resources management, and development of soil conservation methods. in the present study, the temporal changes of soil erosion risk were assessed from 1987 to 2010, based on the revised universal soil loss equation (rusle) using remote sensing (rs) and geographic ...