We describe the karyotype, location of nucleolus-organizing regions (NORs) and heterochromatin composition and distribution in Lepidochitona caprearum (Scacchi, 1836). The examined specimens had 2n=24 chromosomes; the elements of pairs 1-4 were metacentric, subtelocentric those of the fifth pair, telocentric the elements of other pairs. NOR-FISH, Ag-NOR- and CMA3 banding showed NORs localized o...

By January 2005, clonal chromosomal aberrations identified by various banding techniques had been reported in more than 47,000 human neoplasms (Mitelman et al., 2005a). An increasing number of the acquired abnormalities have now also been studied by various fluorescence in situ hybridization techniques, which have provided a new and powerful tool to identify abnormal chromosomes and to visualiz...

G-banding of chromosome metaphase preparations derived from haemic cells of healthy individuals and from patients with acute myeloid leukaemia was performed with the aid of trypsin, papain, and pretreatment of the chromosome spreads with emulphogene before proteolytic digestion. Papain digestion revealed more distinguishable bands than did trypsin digestion. Pretreatment of the chromosome sprea...

By January 2005, clonal chromosomal aberrations identified by various banding techniques had been reported in more than 47,000 human neoplasms (Mitelman et al. 2005a). An increasing number of the acquired abnormalities have now also been studied by various fluorescence in situ hybridization techniques, which have provided a new and powerful tool to identify abnormal chromosomes and to visualize...

A 12 year old girl referred for chromosome analysis because of short stature was found to have karyotype mos 45,X/46,X,+mar. The marker chromosome was observed in 58% of her blood lymphocytes. It was a small, pale staining, spherical fragment with GTL banding and showed faint differentiation along its length with CBG banding. DNA analysis using Y specific probes showed the absence of the testic...

Cytogenetic studies from case 1 included cultures of peripheral blood and skin fibroblasts. Karyotypes from both cultures showed trisomy D(47,XX, + D). Giemsa banding of cells from the skin fibroblast culture, using a modification of the method of Seabright (1971), showed 47,XX,+ 13 (Fig. 3). Chromosome analysis of peripheral leucocytes of the parents were normal. In case 2 the karyotype from a...

The karyotype and other chromosomal characteristics the crucian carp (Carassius carassius (Linnaeus, 1758)) were revealed by means of conventional banding protocols (C, CMA3, AgNOR). The diploid chromosome number (2n) in this species was 100. Its karyotype was composed of 10 pairs of metacentric, 18 pairs of submetacentric and 22 pairs of subtelo- to acrocentric chromosomes without any microchr...

An idiogram construction following chromosome measurements is a versatile tool for cytological, cytogenetic and phylogenetic studies. The information on chromosome length, centromere index and position of cytogenetic landmarks along with modern techniques (e.g. genomic and fluorescence in situ hybridization, banding, chromosome painting) can help to shed light on genome constitution, chromosome...

The distribution of accessible antigenic sites in the chromosomal protein high mobility group one (HMG-1) in Chironomus thummi polytene chromosomes is visualized by immunofluorescence. The results indicate that (a) HMG-1 is distributed in a distinct banding pattern along the entire length of the chromosomes; (b) the banding pattern obtained with fluorescent antibody does not strictly correspond...

A comparative chromosome banding analysis of Chironomussolitus Linevich & Erbaeva, 1971 and Chironomusanthracinus Zetterstedt, 1860 from East Siberia (Lakes Baikal, Gusinoe, Arakhley and Irkutsk Reservoir) showed close similarity of banding sequences. Chironomussolitus differs from Chironomusanthracinus in one species-specific sequence of arm B. Arms C (43%) and D (30%) had inversion banding se...