A simple, Accurate, precise method was developed for the simultaneous estimation of Olmesartan Medoxomil and Hydrochlorothiazide in Tablet dosage form. Chromatogram run through Inertsil -ODS C18,250 x 4.6 mm, 5m. Mobile phase containing Methanoland Bufferin ratio 80:20 pumped column at a flow rate 10ml/min. Optimized wavelength Hydrochlorothiazidewas 260nm. Retention time Medoxomiland were foun...

prolonged local consumption of anethum graveolens leaves, as an antihyperlipimic and antihypercholesterolemic agent, has been associated in some patients with skin disorders such as darkening and pigmentation mainly in the exposed areas such as face and hands.anethum graveolens belongs to the umbelliferae family which is belived to be rich in photoactive furocoumarins. an oraganic extract of th...

In this study, inulooligosaccharides (F(n)-type inulin) resulting from the endo-inulinase hydrolysis of globe artichoke inulin were purified and characterized. The aim was to produce F(n) oligomer standards with the intention of identifying them in the complex inulin chromatogram. Inulin was extracted from globe artichoke and presented a high average degree of polymerization (DP) of about 80 as...

Flavonoids are a class of natural phenolic compounds that show antioxidant properties. Besides the known mechanisms of action of flavonoids (binding/inactivation of free radicals and other reactive oxygen species) that determine this effect, an important factor is their ability to bind transition metal ions. In this paper, we used a HPLC method with a prechromatographic reaction of a sample wit...

The thymidine kinases extracted from the spleen of mice infected with Friend virus and from Yoshida sarcoma in rats were separated into two active peaks by diethylaminoethyl cellulose column chromatography, while those of normal tissues have been found to consist of only the first peak (P-1). The second peak (P-II) was also found in the enzyme from the extract of the spleen when the animals wer...

An improved mass defect filter (MDF) method employing both drug and core structure filter templates was applied to the processing of high resolution LC/MS data for the detection and structural characterization of oxidative metabolites with mass defects similar to or significantly different from those of the parent drugs. The effectiveness of this approach was investigated using nefazodone as a ...

case n > 3. In a related field, that of the natural polyenes, the C40-carotenoids, methods have been used in some laboratories2 for the preparation of stereoisomers, viz., reversible isomerization by refiuxing solutions, by iodine or HCl catalysis, and by melting crystals. Irradiation can also be effective. As the adsorption affinity of stereoisomers varies with the configuration, they can be s...

High performance size exclusion chromatography (HPSEC) with ultraviolet absorbance (UVA) detection has been widely utilized to estimate the molecular weight (MW) and MW distribution of natural organic matter (NOM). However, the estimation of MW with UVA detection is inherently inaccurate because UVA at 254 nm only detects limited components (mostly pi bonded molecules) of NOM, and the molar abs...

Introduction: The Hemoglobinopathies are one of the major public health problems in tribal predominant population. Objective: To estimate the seroprevalence of Sickle Cell Anemia and Thalassemia in suspected cases of genetic disorders by using HPLC retention time chromatogram method in tribal predominant population, Ranchi, Jharkhand. Methods: All Blood sample of suspected cases of genetic diso...

The technique of Hadamard transform was successfully coupled with GC/nonresonant multiphoton ionization/TOFMS, for the first time. 1,4-Dichlorobenzene and the fourth harmonic generation (266 nm) of a Nd:YAG laser were employed as a model sample and an ionization laser, respectively. A Hadamard-injector coupled with a capillary-based supersonic jet nozzle (capillary-injector) was also developed ...