Members of the Mitis group of streptococci possess teichoic acids (TAs) as integral components of their cell wall that are unique among Gram-positive bacteria. Both, lipoteichoic (LTA) and wall teichoic acid, are formed by the same biosynthetic pathway, are of high complexity and contain phosphorylcholine (P-Cho) residues. These residues serve as anchors for choline-binding proteins (CBPs), som...

The snake venom protease ecarin from Echis carinatus was expressed in stable transfected CHO-S cells grown in animal component free cell culture medium. Recombinant ecarin (r-ecarin) was secreted from the suspension adapted Chinese Hamster Ovary (CHO-S) host cells as a pro-protein and activation to the mature form of r-ecarin occurred spontaneously during continued incubation of the cell cultur...

Longitudinal (T1) and apparent transverse relaxation times (T2) of choline-containing compounds (Cho), creatine/phosphocreatine (Cr/PCr), and N-acetyl aspartate (NAA) were measured in vivo in human brain at 4 Tesla. Measurements were performed using a water suppressed stimulated echo pulse sequence with complete outside volume presaturation to improve volume localization at short echo times. T1...

BACKGROUND The optimization of protein production is a complex and challenging problem in biotechnology. Different techniques for transcription, translation engineering and the optimization of cell culture conditions have been used to improve protein secretion, but there remain many open problems involving post-translational modifications of the secreted protein and cell line stability. RESUL...

TURN EXPERIMENT TO TEST QUASI-PANSEPERMIA HYPOTHESIS ONBOARD THE ISS-KIBO EXPOSED FACILITY. H. Yano, A. Yamagishi, H. Hashimoto, S. Yokobori, K. Kobayashi, H. Yabuta, H. Mita, M. Tabata, H. Kawai, M. Higashide, K. Okudaira, S. Sasaki, E. Imai, Y. Kawaguchi, Y. Uchibori, S. Kodaira and the Tanpopo Project Team, JAXA/Institute of Space and Astronautical Science (3-1-1 Yoshinodai, Chuo-ku, Sagamih...

INTRODUCTION The aim of the present study was to investigate the effect of ingesting a glucose plus fructose drink compared with a glucose-only drink (both delivering carbohydrate at a rate of 1.8 g.min(-1)) and a water placebo on endurance performance. METHODS Eight male trained cyclists were recruited (age 32 +/- 7 yr, weight 84.4 +/- 6.9 kg, .VO(2max) 64.7 +/- 3.9 mL.kg(-1).min(-1), Wmax 3...

begin{abstract} in this paper, we introduce an iterative method for amenable semigroup of non expansive mappings and infinite family of non expansive mappings in the frame work of hilbert spaces. we prove the strong convergence of the proposed iterative algorithm to the unique solution of a variational inequality, which is the optimality condition for a minimization problem. the results present...

Nogalamycin is an anthracycline antibiotic which was mark edly cytotoxic in vitro and was active against several tumor systems in vivo. We compare here the lethality of several nogalamycin analogs against Chinese hamster ovary (CHO), mouse leukemia (L1210), and mouse melanoma (B16) cells in culture. 7-con-O-Methylnogarol (7-con-OMEN) was the most lethal of all the analogs tested. Thus, for CHO ...

Relatively little is known about the mechanisms used by somatic cells to regulate the replication of the centrosome complex. Centrosome doubling was studied in CHO cells by electron microscopy and immunofluorescence microscopy using human autoimmune anticentrosome antiserum, and by Northern blotting using the cDNA encoding portion of the centrosome autoantigen pericentriolar material (PCM)-1. C...

The purpose was to determine the effect of carbohydrate (CHO) mouth rinse on maximal voluntary contraction (MVC) and neuromuscular output in a fatigued state. It was hypothesized that CHO mouth rinse would potentiate torque output in a fatigued state. In a double-blind, cross-over design, 12 competitive male athletes (9 rowers, 1 cyclist, 1 runner and 1 volleyball player) initially performed 3 ...