Degenerative disc disease has been implicated as a major component of spine pathology. However, although biological repair of the degenerate disk would be the ideal treatment, there is no universally accepted scaffold for tissue engineering of the intervertebral disk. To help remedy this, we investigated the gelation kinetics of various concentrations (2.5 to 10%) of two water-soluble chitosan ...

This study aims to evaluate the effect of chitosan (CS) scaffold, alone, and potential synergistic when impregnated with simvastatin (SIM), on immortalized human bone-marrow mesenchymal stem cells (hbMMSCs) compared CollaCote (CL). CS scaffolds were fabricated seeded hBMMSCs. Samples divided into control groups (negative no added material positive CL added) four experimental groups: CS/SIM 0.01...

Magnetic 'nanofactories', for localized manufacture and signal-guided delivery of small molecules to targeted cell surfaces, are demonstrated. They recruit nearby raw materials for synthesis, employ magnetic mobility for capture and localization of target cells, and deliver molecules to cells triggering their native phenotypic response, but with user-specified control. Our nanofactories, which ...

Polymeric nanofibers that mimic the structure and function of the natural extracellular matrix (ECM) are of great interest in tissue engineering as scaffolding materials to restore, maintain or improve the function of human tissues. Electrospinning has been recently developed as an effective technique for nanofiber fabrication. In our study, the electropsinning method is used to create hybrid n...

Chondral or osteochondral defects are still controversial problems in orthopedics. Here, chondrocytes labeled with magnetic nanoparticles were cultivated on a biphasic, type II collagen-chitosan/poly(lactic-co-glycolic acid) scaffold in an attempt to develop cultures with trackable cells exhibiting growth, differentiation, and regeneration. Rabbit chondrocytes were labeled with magnetic nanopar...

Repair of deep burn by use of the dermal equivalent relies strongly on the angiogenesis and thereby the regeneration of dermis. To enhance the dermal regeneration, in this study plasmid DNA encoding vascular endothelial growth factor-165 (VEGF-165)/N,N,N-trimethyl chitosan chloride (TMC) complexes were loaded into a bilayer porous collagen-chitosan/silicone membrane dermal equivalents (BDEs), w...

Huafeng Sun, Xingang Wang, Weijie Yu, Xinlei Hu, Chuangang You, Hang Hu, Chunmao Han* Department of Burn, Second Affiliated Hospital of Zhejiang University, Hangzhou, 310009, China *Corresponding Author Email: [email protected] Introduction Local delivery of GM-CSF (granulocyte-macrophage colony-stimulating factor) has been has been used for treating human chronic skin ulcers and deep secondd...

Cell-based tissue engineering using scaffolds provides a promising option for the repair of bone tissue damage caused by trauma or aging-related degeneration such as osteoporosis. In this study, a porous 3D scaffold was used to support the differentiation process of rat adipose -derived stem cells (ADSCs) into osteoblasts in vitro. The scaffold was made with chitosan, gelatin and chondroitin. I...

Chitosan-collagen scaffolds were produced by freeze-drying method and coated with a thermoresponsive polymer poly(N, N-diethylacrylamide) (PDEAAm) to confer them temperature sensitivity using a non residue technology, the supercritical carbon dioxide (scCO2). All native and coated scaffolds were characterized by SEM, intrusion mercury porosimetry, swelling, biodegradability and mechanical analy...

Electrospinning of collagen and chitosan blend solutions in a 1,1,1,3,3,3-hexafluoroisopropanol/trifluoroacetic acid (v/v, 90/10) mixture was investigated for the fabrication of a biocompatible and biomimetic nanostructure scaffold in tissue engineering. The morphology of the electrospun collagen-chitosan nanofibers was observed by scanning electron microscopy (SEM) and stabilized by glutaralde...