Instability and inadequate biodistribution of double-stranded RNA are major drawbacks to the clinical use of RNA interference. This work compares chemical modification and nanoparticle formulation as strategies to improve the systemic delivery of small interfering RNA (siRNA). Variable levels of chemical modified siRNA, either naked or within nanoparticle, were intravenously injected into mice ...

Chitosan nanoparticles have been prepared using an ionic gelation method. The effects of freezedrying, cryoprotectant (D-trehalose) utilisation and ultrasonication time on the mean particle size, size distribution and stability of nanoparticles were investigated. The size distribution results indicated that freeze-drying caused the particles to agglomerate. Addition of the cryoprotectant and ul...

Neurodegeneration is characterized by progressive loss of structure and function of neurons. Several therapeutic methods and drugs are available to alleviate the symptoms of these diseases. The currently used delivery strategies such as implantation of catheters, intracarotid infusions, surgeries, and chemotherapies are invasive in nature and pose a greater risk of postsurgical complications, w...

Chitosan is the deacetylated form of chitin and used in numerous applications. Because it is a good dispersant for metal and/or oxide nanoparticle synthesis, chitosan and its derivatives have been utilized as coating agents for magnetic nanoparticles synthesis, including superparamagnetic iron oxide nanoparticles (SPIONs). Herein, we demonstrate the water-soluble SPIONs encapsulated with a hybr...

A novel selective and sensitive electrochemical sensor for insulin detection has been fabricated and investigated. The electrochemical sensor was made from a mixture of silica gel, chitosan and nickel hydroxide (Ni(OH)2) nanoparticles, which was mounted on a silver wire and covered by a glass tube (silica gel/chitosan/Ni(OH)2 nanoparticles paste electrode). The sensor was characterized using cy...

A DNA/chitosan-Fe₃O₄ magnetic nanoparticle bio-complex film was constructed for the immobilization of horseradish peroxidase (HRP) on a glassy carbon electrode. HRP was simply mixed with DNA, chitosan and Fe₃O₄ nanoparticles, and then applied to the electrode surface to form an enzyme-incorporated polyion complex film. Scanning electron microscopy (SEM) was used to study the surface features of...

Abstract This study endeavors to augment the antibacterial and anti‐biofouling properties of thin‐film composite polyamide membranes by subjecting them modification with chitosan silver nanoparticle materials through UV‐induced graft polymerization technique. Various surface assessment methods were employed, efficiency membrane performance was demonstrated flux retention filtration process, whi...

context: over the last few decades, extensive efforts have been made worldwide to develop nanomedicine delivery systems, especially via oral route for antidiabetic drugs. absorption of insulin is hindered by epithelial cells of gastrointestinal tract, acidic gastric ph and digestive enzymes. evidence acquisition: recent reports have identified and explained the beneficial role of several struct...

An eco-friendly and simple approach was carried out for the synthesis of silver-chitosan nanocomposites using Azadirachta indica fluconazole-mediated aqueous extract. This extract acted as a reducing agent well capping green silver nanoparticles. Chitosan nanoparticles on other hand were synthesized from deacetylation chitin matrix. To confirm nanoparticle synthesis, UV- A visible spectrophotom...