Objective: The induction of neural stem cells (NSCs) from human induced pluripotent stem cells (hiPSCs) developed into an important strategy to derive patient-specific neuronal and glial cells. Several neural differentiation protocols have been developed mainly involving laborious experimentation such as embryoid body (EB) formation or manual neural rosette isolation. The aim of this study is t...

Endothelial cells (ECs) sense and respond to fluid flow regulate immune cell trafficking in all organs. Despite sharing the same mesodermal origin, ECs exhibit heterogeneous tissue-specific characteristics. Human pluripotent stem (hPSCs) can potentially be harnessed capture this heterogeneity further elucidate endothelium behavior satisfy need for increased accuracy breadth of disease models th...

Human pluripotent stem cells (PSCs) are a promising source of cells for applications in regenerativemedicine.Directed differentiation of PSCs into specialized cells such as spinal motoneurons or midbrain dopamine (DA) neurons has been achieved. However, the effective use of PSCs for cell therapy has lagged behind.Whereasmouse PSCderived DA neurons have shown efficacy in models of Parkinson’s di...

•Endocardial cells can be generated from hPSC-derived cardiovascular mesoderm•BMP10 is required for specification and maintenance of human endocardial cells•Human induce a trabecular fate in cardiomyocytes•hPSC-derived generate valvular interstitial-like The embryonic endocardium essential early heart development as it functions to myocardium, the first tissue form, source that make up valves p...

•WNT regulates rostrocaudal neural tube patterning via a tristable network motif.•Blocking hindbrain fate may improve generation of dopaminergic neurons from hPSCs.•Combined and dorsoventral model correctly patterns 3D tube.•Models in vivo processes can be built modularly using data diverse sources. Rostrocaudal the is defining event vertebrate brain development. This process driven by morphoge...

Renin production by the kidney is of vital importance for salt, volume, and blood pressure homeostasis. The lack human models hampers investigation into regulation renin its relevance physiology. To develop such a model, we used induced pluripotent stem cell–derived organoids to study role renin-angiotensin system in kidney. Extensive characterization revealed kidney-specific cell populations c...

Jmjd2/Kdm4 H3K9-demethylases cooperate in promoting mouse embryonic stem cell (ESC) identity. However, little is known about their importance at the exit of ESC pluripotency. Here, we uncover that Jmjd2c facilitates this process by stabilizing the assembly of Mediator-Cohesin complexes at lineage-specific enhancers. Functionally, we show that Jmjd2c is required in ESCs to initiate appropriate g...

Extracellular vesicles (EVs) can modulate microenvironments by transferring biomolecules including RNAs and proteins derived from releasing cells to target cells. To understand the molecular mechanisms maintaining the neural stem cell (NSC) niche through EVs, a new transgenic (Tg) rat strain that can release human CD63-GFP expressed EVs from the NSCs was established. Human CD63-GFP expression w...