Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO) cells were transfected with pEGFP-N1-Fyn. The expression of Fyn mRNA and proteins was monitored by reverse transcription-PCR and Western blotting. Additionally, transfecte...

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

We report the synthesis and radioligand binding analysis of a series of naphthalenic melatonin receptor ligands, N-[2-(7-alkoxy-2-methoxy-1-naphthyl)ethyl]propionamide. This series of ligands exhibits subpicomolar binding affinity to both MT1 and MT2 melatonin receptors expressed in chinese hamster ovary (CHO) cells.

Chinese hamster ovary (CHO) cells is one of the most widely used production host for the commercial production of biopharmaceuticals product. They have been extensively studied and developed, and today provide a stable platform for producing monoclonal antibodies and recombinant proteins. This study focused on the production of recombinant protein in suspension culture of CHO cells in spinner f...

introduction: neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. also, neurotropic factors are growth factors like neurotrophins that help neurons survive. moreover, neurotrophins differentiate between progenitor cells so that neurons are formed. despite the fact that the majority of mammalian brain neurons are produced p...

BACKGROUND Xenopus egg extracts initiate replication specifically at the Chinese Hamster Ovary (CHO) cell dihydrofolate reductase (DHFR) origin with CHO G1-phase nuclei as a substrate, providing that these nuclei have intact nuclear envelopes and are isolated from cells that have passed through a distinct transition (origin decision point; ODP) early in G1-phase. With intact pre-ODP nuclei, or ...

A somatic cell mutant of the Chinese hamster ovary (CHO)-K1 cell auxotrophic for mevalonic acid has been isolated by means of the bromodeoxyuridine-visible light technique. This mutant can incorporate labeled mevalonate but not labeled acetate into cholesterol and, thus, is apparently defective in mevalonate biosynthesis. The mutant is recessive with respect to the parental cell phenotype. Asse...

Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed tem...

ABBREVIATIONS B103: Brain cell line 103, CDC: Centers for Disease Control and Prevention, CHO: Chinese Hamster Ovary, CMI: Cell mediated immunity, CNS: Central nervous system, CSF: Cerebro-spinal fluid, E6:Vero monkey kidney cell culture, HLF: Human lung fibroblast, HMG: High mobility group protein, HSP70: Heat shock protein 70, IFA: Immunoflurescent assay, ITS: Internal transcribed spacers, kD...

Many purified plant lectins specifically interact with the carbohydrate moieties of glycoproteins and glycolipids at the cell surface and are cytotoxic (1, 2). Lectinresistant mutants o f animal cells have been isolated which have alterations in cell membrane molecules (3-6). It was found that a series of iectin-resistant mutants we isolated were much more sensitive to the cytotoxic activity o ...